IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)
Recombinant Ebola virus EBOV (subtype Sudan, strain Gulu) VP40 / Matrix protein VP40 Protein (Catalog#40447-V07E)
Preparation
Produced in rabbits immunized with purified, recombinant EBOV Ebola virus EBOV (subtype Sudan, strain Gulu) VP40 / Matrix protein VP40 (Catalog#40447-V07E; YP_138522.1; Val31-Lys326). Ebola virus EBOV (subtype Sudan, strain Gulu) VP40 / Matrix protein VP40 specific IgG was purified by EBOV Ebola virus EBOV (subtype Sudan, strain Gulu) VP40 / Matrix protein VP40 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Image / Experimental Information
Image 1 Description
Anti-Ebola virus EBOV (subtype Sudan, strain Gulu) VP40 / Matrix protein VP40 rabbit polyclonal antibody at 1:1000 dilution. Sample:Ebola virus EBOV (subtype Sudan, strain Gulu) VP40 / Matrix protein VP40 Recombinant Protein Lane A: 10ng Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions.
Image 1 Alt Text
EBOV EBOV-VP40/Matrix protein VP40 Western blot (WB) 14948
title: The C-terminus of Sudan ebolavirus VP40 contains a functionally important CX n C motif, a target for redox modifications. authors: Anke-Dorothee Werner; Martin Schauflinger; ...; Stephan Becker journal: Structure (London, England : 1993) date: 2023-09-12 pmid: 37392738 doi: 10.1016/j.str.2023.06.004 cited_by_count: 3 techniques: Recombinant; Transfection; Virus bioz_score: 91
title: RNF185 regulates proteostasis in Ebolavirus infection by crosstalk between the calnexin cycle, ERAD, and reticulophagy. authors: Jing Zhang; Bin Wang; ...; Yong-Hui Zheng journal: Nature Communications date: 2022-10-14 pmid: 36224200 doi: 10.1038/s41467-022-33805-9 cited_by_count: 18 techniques: Membrane; Nucleic Acid Electrophoresis; Saline; Western Blot bioz_score: 90