| Catalog No. | Size | Price (USD) |
| 40094-R301-20 | 20 µL | $118.49 |
| 40094-R301-50 | 50 µL | $222.17 |
| 40094-R301-100 | 100 µL | $370.28 |
General Information
| Product name | Ebola virus EBOV (Subtype Sudan, strain Gulu) Glycoprotein / GP1 (mucin domain deleted) Antibody, Rabbit MAb |
| Validated applications | ELISA (FAQ Protocol) |
| Application notes | IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.) |
| Specificity | Ebola virus EBOV (Subtype Sudan, strain Gulu) Glycoprotein / GP1 (mucin domain deleted) |
| Immunogen | Recombinant Sudan ebolavirus EBOV-G / Glycoprotein Protein (Catalog#40094-V08H) |
| Preparation | This antibody was obtained from a rabbit immunized with purified, recombinant Sudan ebolavirus EBOV-G / Glycoprotein (rSudan ebolavirus EBOV-G / Glycoprotein; Catalog#40094-V08H; Q7T9D9; Met1-Asp320). |
| Source | Monoclonal Rabbit IgG Clone #301 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Background Information
| Description | The fourth gene of the EBOV genome encodes a 16-kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD); the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry; the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm. |
| References |
|
title: Serological Detection of Ebola Virus Exposures in Native Non-human Primates of Southern Nigeria
authors: B.N. Ogunro; B.O. Olugasa; ...; H. Niphuis
journal: Journal of Epidemiology and Global Health
date: 2018-01-01
doi: 10.1016/j.jegh.2018.05.001
cited_by_count: 5
techniques: Cell Culture; Enzyme-linked Immunosorbent Assay; Irradiation; Positive Control; Purification
bioz_score: 90
