Reacts with: Human, Species predicted to react based on 100% sequence homology: Mouse, rat
Specificity
Human Species predicted to react based on 100% sequence homology: Mouse, rat
Immunogen
A synthetic phosphopeptide corresponding to residues around Ser51 of human Phospho-eIF2α.
Preparation
This antibody was obtained from a rabbit immunized with a synthetic phosphopeptide corresponding to residues around Ser51 of human Phospho-eIF2α.
Source
Monoclonal Rabbit IgG Clone #0011
Purification
Protein A
Formulation
10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with Calyculin A (100nM, 30 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-eIF2α (Ser51) rabbit monoclonal Antibody (Cat# 110457-R0011) at 1:1000 dilution. (Validation Experiment)
Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with Calyculin A (100nM, 30 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-eIF2α (Ser51) rabbit monoclonal Antibody (Cat# 110457-R0011) at 1:1000 dilution. (Validation Experiment)
Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with Calyculin A (100nM, 30 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-eIF2α (Ser51) rabbit monoclonal Antibody (Cat# 110457-R0011) at 1:1000 dilution. (Validation Experiment)