| Catalog No. | Size | Price (USD) |
| 109892-T32-50 | 50 µL | $147.96 |
| 109892-T32-100 | 100 µL | $251.64 |
| 109892-T32-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-EIF5 Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human EIF5 |
| Immunogen | A synthetic peptide corresponding to the C-terminus of the Human EIF5 |
| Preparation | Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human EIF5, and purified by antigen affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | PBS, pH7.0 with 0.03% Proclin300 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-EIF-5 Antibody; Anti-EIF-5A Antibody
Image / Experimental Information
| Image 1 Description | Anti-EIF5 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: K562 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:49 kDa |
| Image 1 Alt Text | Human EIF5 Western blot (WB) 35335 |
| Image 1 URL | Source image |
Background Information
| Full Name | eukaryotic translation initiation factor 5 |
| Description | EIF-5A, also known as EIF5, functions in start site selection as a GTPase accelerating protein (GAP) for the eukaryotic translation initiation factor (eIF) 2•GTP•tRNA ternary complex within the ribosome-bound pre-initiation complex. In protein synthesis initiation, eIF2 functions in its GTP-bound state to deliver initiator methionyl-tRNA to the small ribosomal subunit and is necessary for protein synthesis in all cells. EIF-5A stabilizes the binding of GDP to eIF2 and is therefore a bi-functional protein that acts as a GDP dissociation inhibitor (GDI). EIF-5A also interacts with eIF1 and eIF3 and binds the eIF2-GTP/Met-tRNA ternary complex along with the 40S ribosome subunit. |
| Related Pathways |
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| References |
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