IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)
Specificity
Monkeypox Virus (MPXV) Envelope protein E8L Has cross-reactivity in WB and ELISA with Monkeypox Virus (MPXV) (Clade Ib) Envelope protein E8L Protein (Cat# 41042-V08H)
Immunogen
Recombinant Monkeypox Virus (MPXV) Envelope protein E8L Protein (Catalog# 40890-V08B)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Monkeypox Virus (MPXV) Envelope protein E8L (Catalog# 40890-V08B; QNP13700.1; Met1-Thr275). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #16
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Image / Experimental Information
Image 1 Description
Anti-MPXV-E8L mouse monoclonal antibody at 1:10000 dilution. Lane A: Monkeypox Virus (MPXV) Envelope protein E8L Protein (Cat# 40890-V08B) (10ng) Secondary Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions.
Anti-MPXV-E8L mouse monoclonal antibody at 1:10000 dilution. Lane A: Monkeypox Virus (MPXV) Envelope protein E8L Protein (Cat# 40890-V08B) (10ng) Secondary Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions.