Produced in rabbits immunized with E. coli-derived Human FAM198A fragment, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
PBS, pH7.0 with 0.03% Proclin300
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.
Image / Experimental Information
Image 1 Description
Immunofluorescence staining of FAM198A in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human FAM198A polyclonal antibody (dilution ratio 1:200) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
GASK1A (Golgi Associated Kinase 1A) is located on human chromosome 3p22.1. FAM198A is a member of four-jointed protein kinases, a secreted protein kinase family. The FAM198A (Also known as C3orf41) gene is conserved in chimpanzee, Rhesus monkey, dog, mouse, and rat. 210 organisms have orthologs with human gene FAM198A. The GASK1A protein is broadly expressed in gall bladder, endometrium and other tissues. Structurally, the encoded protein is reported to be 63637 Da in mass. GASK1A (Golgi Associated Kinase 1A) is associated with some diseases, such as Lipodystrophy, Congenital Generalized, Type 4. An important paralog of this gene is GASK1B.
References
Wei Z, et al. (2018) Fam198a, a member of secreted kinase, secrets through caveolae biogenesis pathway. Acta Biochim Biophys Sin (Shanghai) 50 (10): 968-975.
-HD13AP1234-200-U2OS-IF.png){kind=link}