Produced in rabbits immunized with E. coli-derived Human FARSA fragment, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
PBS, pH7.0 with 0.03% Proclin300
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.
Anti-FARSA rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lane B: NIH-3T3 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:58 kDa Observed band size:58 kDa
Anti-FARSA rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lane B: NIH-3T3 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:58 kDa Observed band size:58 kDa
Anti-FARSA rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lane B: NIH-3T3 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:58 kDa Observed band size:58 kDa
Anti-FARSA rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lane B: NIH-3T3 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:58 kDa Observed band size:58 kDa
Anti-FARSA rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lane B: NIH-3T3 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:58 kDa Observed band size:58 kDa
The charging of tRNA molecules with phenylalanine in the cytoplasm is accomplished via a tetrameric enzyme that contains two catalytic subunits encoded by phenylalanyl-tRNA synthetase alpha (FARSA; MIM# 602918) and two regulatory subunits encoded by phenylalanyl-tRNA synthetase beta (FARSB; MIM# 609690). Aminoacyl-tRNA synthetases (ARSs) are ubiquitously expressed enzymes that ligate tRNA molecules to cognate amino acids, which is the first step of protein translation. Genetic mutations that cause dysfunction or absence of ARSs result in various neurodevelopmental disorders.
References
Antonellis A, et al. (2018) Compound heterozygosity for loss-of-function farsb variants in a patient with classic features of recessive aminoacyl-trna synthetase-related disease. Hum Mutat 39 (6): 834-840.