| Catalog No. | Size | Price (USD) |
| 207325-T02-50 | 50 µL | $147.96 |
| 207325-T02-100 | 100 µL | $251.64 |
| 207325-T02-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-FBXL12 Antibody, Rabbit Polyclonal |
| Validated applications | ICC/IF (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human FBXL12 |
| Immunogen | E. coli-derived Human FBXL12 fragment |
| Preparation | Produced in rabbits immunized with E. coli-derived Human FBXL12 fragment, and purified by antigen affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | PBS, pH7.0 with 0.03% Proclin300 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-Fbl12 Antibody; Anti-FLJ20188 Antibody
Image / Experimental Information
| Image 1 Description | Immunofluorescence staining of FBXL12 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human FBXL12 polyclonal antibody (dilution ratio 1:200) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm. |
| Image 1 Alt Text | Human FBXL12 Immunofluorescence(IF) 26456 |
| Image 1 URL | Source image |
Background Information
| Full Name | F-box and leucine rich repeat protein 12 |
| Description | The ubiquitin-dependent degradation of ALDH3 mediated by FBXL12 (F box and leucine-rich repeat protein 12) is essential for execution of the differentiation program of trophoblast stem cells (TSCs). FBXL12 is present only in eutherian mammals, and its expression is largely restricted to the placenta during mouse embryogenesis. FBXL12 was found to interact specifically with members of the ALDH3 family and to mediate their polyubiquitylation. Most mice deficient in FBXL12 died during the embryonic or perinatal period probably as a result of abnormal development of the placenta, characterized by impaired formation of the junctional zone. FBXL12 plays a key role in the downregulation of ALDH3 activity in TSCs and thereby initiates trophoblast differentiation during placental development. |
| References |
|
-HD13MA3016-200-U2OS-IF.png){kind=link}