| Catalog No. | Size | Price (USD) |
| 110591-R0113-20 | 20 µL | $103.53 |
| 110591-R0113-100 | 100 µL | $296.07 |
General Information
| Product name | Recombinant Phospho-AKT (Ser473) Antibody, Rabbit Monoclonal |
| Validated applications | WB (FAQ Protocol), IHC-P (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human, Mouse Species predicted to react based on 100% sequence homology: Rat Phospho-AKT (Ser473) |
| Immunogen | A synthetic phosphopeptide corresponding to residues around Ser473 of human AKT. |
| Preparation | The antibody is produced by immunizing rabbits with a synthetic phosphopeptide corresponding to residues around Ser473 of human AKT. |
| Source | Monoclonal Rabbit IgG Clone #0113 |
| Purification | Protein A |
| Formulation | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-AKT Antibody; Anti-PKB Antibody; Anti-PKB-ALPHA Antibody; Anti-PRKBA Antibody; Anti-RAC Antibody; Anti-RAC-ALPHA Antibody
Image / Experimental Information
| Image 1 Description | Immunohistochemical analysis of paraffin-embedded human lung cancer tissue, untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-AKT (Ser473) Antibody, Rabbit Monoclonal (Cat# 110591-R0113) at 1:200 dilution. (Validation Experiment) |
| Image 1 Alt Text | Human AKT1 Immunohistochemistry(IHC) 37380 |
| Image 1 URL | Source image |
| Image 2 Description | Immunohistochemical analysis of paraffin-embedded human lung cancer tissue, untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-AKT (Ser473) Antibody, Rabbit Monoclonal (Cat# 110591-R0113) at 1:200 dilution. (Validation Experiment) |
| Image 2 Alt Text | Human AKT1 Immunohistochemistry(IHC) 37379 |
| Image 2 URL | Source image |
| Image 3 Description | Immunohistochemical analysis of paraffin-embedded human lung cancer tissue, untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-AKT (Ser473) Antibody, Rabbit Monoclonal (Cat# 110591-R0113) at 1:200 dilution. (Validation Experiment) |
| Image 3 Alt Text | Human AKT1 Western blot (WB) 37297 |
| Image 3 URL | Source image |
| Image 4 Description | Immunohistochemical analysis of paraffin-embedded human lung cancer tissue, untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-AKT (Ser473) Antibody, Rabbit Monoclonal (Cat# 110591-R0113) at 1:200 dilution. (Validation Experiment) |
| Image 4 Alt Text | Human AKT1 Western blot (WB) 37296 |
| Image 4 URL | Source image |
| Image 5 Description | Immunohistochemical analysis of paraffin-embedded human lung cancer tissue, untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-AKT (Ser473) Antibody, Rabbit Monoclonal (Cat# 110591-R0113) at 1:200 dilution. (Validation Experiment) |
| Image 5 Alt Text | Human AKT1 Western blot (WB) 36824 |
| Image 5 URL | Source image |
Background Information
| Full Name | v-akt murine thymoma viral oncogene homolog 1 |
| Description | v-akt murine thymoma viral oncogene homolog 1 (AKT1), or protein kinase B-alpha (PKB-ALPHA) is a serine-threonine protein kinase, belonging to the Protein Kinase Superfamily. AKT1 is a major mediator of the responses to insulin, insulin-like growth factor 1 (IGF1), and glucose. AKT1 also plays a key role in the regulation of both muscle cell hypertrophy and atrophy. AKT1 activity is required for physiologic cardiac growth in response to IGF1 stimulation or exercise training. In contrast, AKT1 activity was found to antagonize pathologic cardiac growth that occurs in response to endothelin 1 stimulation or pressure overload. AKT1 selectively promotes physiological cardiac growth while AKT2 selectively promotes insulin-stimulated cardiac glucose metabolism. AKT1 deletion prevented tumor initiation as well as tumor progression, coincident with decreased Akt signaling in tumor tissues. AKT1 is the primary Akt isoform activated by mutant K-ras in lung tumors, and that AKT3 may oppose AKT1 in lung tumorigenesis and lung tumor progression. A number of separate studies have implicated AKT1 as an inhibitor of breast epithelial cell motility and invasion. AKT1 may have a dual role in tumorigenesis, acting not only pro-oncogenically by suppressing apoptosis but also anti-oncogenically by suppressing invasion and metastasis. |
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| References |
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title: Artificial intelligence combined with affinity chromatography discover that 7-Epitaxol promotes autophagy in NSCLC cells by interacting with EGFR: Discovery of novel EGFR antagonist based on DL and CMC.
authors: Haoyun Bai; Tong Wu; ...; Shengli Han
journal: Phytomedicine : international journal of phytotherapy and phytopharmacology
date: 2025-08-26
pmid: 40768807
doi: 10.1016/j.phymed.2025.157127
cited_by_count: 1
techniques: CCK-8 Assay; Modification; Protease Inhibitor; Recombinant
bioz_score: 93
title: Size-optimized simvastatin-loaded TPGS modified lipid nanocapsules for targeting epithelial-to-mesenchymal transition in hepatocellular carcinoma: Role of PTEN/AKT signaling.
authors: Khaled Mahmoud; Mahmoud Teaima; ...; Shady Swidan
journal: Expert opinion on drug delivery
date: 2023-06-25
pmid: 37208857
doi: 10.1080/17425247.2023.2216451
cited_by_count: 4
techniques: Bicinchoninic Acid Protein Assay; Enzyme-linked Immunosorbent Assay
bioz_score: 93
