| Catalog No. | Size | Price (USD) |
| 110848-R0056-20 | 20 µL | $103.53 |
| 110848-R0056-100 | 100 µL | $296.07 |
General Information
| Product name | Phospho-AKT1 (Thr450) Antibody, Rabbit MAb |
| Validated applications | WB (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human, Mouse Species predicted to react based on 100% sequence homology: Rat, Cynomolgus Phospho-AKT1 (Thr450) of P31749 in UniProt. |
| Immunogen | A synthetic phosphopeptide corresponding to residues around (Thr450) of the Human Phospho-AKT1 |
| Preparation | This antibody was obtained from a rabbit immunized with a synthetic phosphopeptide corresponding to residues around (Thr450) of the Human Phospho-AKT1. |
| Source | Monoclonal Rabbit IgG Clone #0056 |
| Purification | Protein A |
| Formulation | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-AKT Antibody; Anti-PKB Antibody; Anti-PKB-ALPHA Antibody; Anti-PRKBA Antibody; Anti-RAC Antibody; Anti-RAC-ALPHA Antibody
Image / Experimental Information
| Image 1 Description | Western blot analysis of extracts from serum-starved NIH 3T3, untreated (line A); treated with PDGFA (Cat# 13081-H07Y) (5 μg/mL, 5 min; +) (line B); treated with PDGFA and λ-phosphatase (line C) using Phospho-AKT (Thr450) rabbit monoclonal Antibody (Cat# 110848-R0056) at 1:1000 dilution. |
| Image 1 Alt Text | Human AKT1 Western blot (WB) 39777 |
| Image 1 URL | Source image |
Background Information
| Full Name | v-akt murine thymoma viral oncogene homolog 1 |
| Description | v-akt murine thymoma viral oncogene homolog 1 (AKT1), or protein kinase B-alpha (PKB-ALPHA) is a serine-threonine protein kinase, belonging to the Protein Kinase Superfamily. AKT1 is a major mediator of the responses to insulin, insulin-like growth factor 1 (IGF1), and glucose. AKT1 also plays a key role in the regulation of both muscle cell hypertrophy and atrophy. AKT1 activity is required for physiologic cardiac growth in response to IGF1 stimulation or exercise training. In contrast, AKT1 activity was found to antagonize pathologic cardiac growth that occurs in response to endothelin 1 stimulation or pressure overload. AKT1 selectively promotes physiological cardiac growth while AKT2 selectively promotes insulin-stimulated cardiac glucose metabolism. AKT1 deletion prevented tumor initiation as well as tumor progression, coincident with decreased Akt signaling in tumor tissues. AKT1 is the primary Akt isoform activated by mutant K-ras in lung tumors, and that AKT3 may oppose AKT1 in lung tumorigenesis and lung tumor progression. A number of separate studies have implicated AKT1 as an inhibitor of breast epithelial cell motility and invasion. AKT1 may have a dual role in tumorigenesis, acting not only pro-oncogenically by suppressing apoptosis but also anti-oncogenically by suppressing invasion and metastasis. |
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