Anti-GAPDH Antibody, Mouse Monoclonal sinobiological 10094-MM13

$143.00
In stock
SKU
10094-MM13
Catalog No.SizePrice (USD)
10094-MM13-5050 µL$147.96
10094-MM13-100100 µL$251.64
10094-MM13-200200 µL$355.32

General Information

Product nameAnti-GAPDH Antibody, Mouse Monoclonal
Validated applicationsWB (FAQ Protocol)
Species reactivityReacts with: Human
SpecificityHuman GAPDH
ImmunogenRecombinant Human GAPDH Protein (Catalog#10094-H07E)
PreparationThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human GAPDH (rh GAPDH; Catalog#10094-H07E; P04406; Met1-Glu335). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
SourceMonoclonal Mouse IgG1 Clone #13
PurificationProtein A
Formulation0.2 μm filtered solution in PBS
ConjugateUnconjugated
FormLiquid
ShippingThis antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
StorageThis antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Synonyms: Anti-CDABP0047 Antibody; Anti-G3PD Antibody; Anti-GAPD Antibody; Anti-HEL-S-162eP Antibody; Anti-OK/SW-cl.12 Antibody

Image / Experimental Information

Image 1 DescriptionAnti-GAPDH mouse monoclonal antibody at 1:1000 dilution Lane A: Hela Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lane D: MCF7 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:36 kDa Observed band size:36 kDa
Image 1 Alt TextHuman GAPDH Western blot (WB) 31586
Image 1 URLSource image

Background Information

Full Nameglyceraldehyde-3-phosphate dehydrogenase
DescriptionGlyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.
Research Areas
  • Adaptor Proteins
References
  1. Hara MR, et al . (2006) Neuroprotection by pharmacologic blockade of the GAPDH death cascade. PNA. 103 (10): 3887-9.
  2. Hara MR, et al . (2006) GAPDH as a sensor of NO stress.Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1762 (5): 502-9.
  3. Tarze A, et al . (2007) GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilizationGAPDH and apoptosis. Oncogene. 26: 2606-20.
  4. Yi MK, et al . (2000) Functional Significance of the Interaction of Hepatitis A Virus RNA with Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH): Opposing Effects of GAPDH and Polypyrimidine Tract Binding Protein on Internal Ribosome Entry Site Function. Journal of Virology. 74 (14) : 6459-68.

title: Chemically modified tRNA enhances the translation capacity of mRNA rich in cognate codons
authors: Liangzhen Dong; Jiayu Wang; Qing Xia
journal: Nature Communications
date: 2025-08-22
pmid: 40846860
cited_by_count: 1
techniques: Biomarker Discovery
bioz_score: 93

title: Neuregulin 4 (Nrg4) cooperates with melatonin to regulate the PRL expression via ErbB4/Erk signaling pathway as a potential prolactin (PRL) regulator.
authors: Wen-Wen Lin; Guan-Yong Ou; ...; Wei-Jiang Zhao
journal: Journal of Cellular Biochemistry
date: 2024-03-11
pmid: 38465779
doi: 10.1002/jcb.30551
cited_by_count: 2
techniques: Software
bioz_score: 92

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