Recombinant Anti-GFP Tag Antibody, Rabbit Monoclonal sinobiological 13105-R208

$857.00
In stock
SKU
13105-R208
Catalog No.SizePrice (USD)
13105-R208-11 mL$888.67
13105-R208-55 mL$2,666.00
13105-R208-2020 µL$118.49
13105-R208-5050 µL$148.11
13105-R208-100100 µL$236.98
13105-R208-500500 µL$592.44

General Information

Product nameRecombinant Anti-GFP Tag Antibody, Rabbit Monoclonal
Validated applicationsWB (FAQ Protocol), ELISA (FAQ Protocol), IP (FAQ Protocol)
Species reactivityReacts with: Aequorea victoria
SpecificityRecognize GFP, EGFP, GFPSpark® Has cross-reactivity in ELISA and WB with Aequorea victoria GFP Protein (Cat# 13105-S07E) EGFP / Enhanced Green Fluorescent Protein Aequorea victoria Green fluorescent protein /enhanced GFP GFPSpark TM (Cat# 69001-S08E)
ImmunogenRecombinant Aequorea victoria GFP protein (Catalog#13105-S07E)
PreparationThis antibody was obtained from a rabbit immunized with purified, recombinant Aequorea victoria GFP (Catalog#13105-S07E; AAB65663; Ser2-Lys238).
SourceMonoclonal Rabbit IgG Clone #208
PurificationProtein A
Formulation0.2 μm filtered solution in PBS
ConjugateUnconjugated
FormLiquid
ShippingThis antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
StorageThis antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Image / Experimental Information

Image 1 DescriptionAnti-GFP Tag was immunoprecipitated using: Lane A: GFP transfected E.coli cell lysate (0.5mg) Lane B: GFP transfected 293 cell lysate (0.5mg) Anti-GFP Tag mouse monoclonal antibody at 1/500-1/2000 dilution and 60μg of Immunomagnetic beads Protein G. Primary antibody: Anti-GFP Tag rabbit monoclonal antibody, at 1/1000-1/5000 dilution Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions.
Image 1 Alt TextAequorea victoria Green fluorescent protein / GFP Immunoprecipitation(IP) 8158
Image 1 URLSource image
Image 2 DescriptionAnti-GFP Tag was immunoprecipitated using: Lane A: GFP transfected E.coli cell lysate (0.5mg) Lane B: GFP transfected 293 cell lysate (0.5mg) Anti-GFP Tag mouse monoclonal antibody at 1/500-1/2000 dilution and 60μg of Immunomagnetic beads Protein G. Primary antibody: Anti-GFP Tag rabbit monoclonal antibody, at 1/1000-1/5000 dilution Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions.
Image 2 Alt TextAequorea victoria Green fluorescent protein / GFP Western blot (WB) 13529
Image 2 URLSource image
Image 3 DescriptionAnti-GFP Tag was immunoprecipitated using: Lane A: GFP transfected E.coli cell lysate (0.5mg) Lane B: GFP transfected 293 cell lysate (0.5mg) Anti-GFP Tag mouse monoclonal antibody at 1/500-1/2000 dilution and 60μg of Immunomagnetic beads Protein G. Primary antibody: Anti-GFP Tag rabbit monoclonal antibody, at 1/1000-1/5000 dilution Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions.
Image 3 Alt TextAequorea victoria GFP Tag Western blot (WB) 34325
Image 3 URLSource image
Image 4 DescriptionAnti-GFP Tag was immunoprecipitated using: Lane A: GFP transfected E.coli cell lysate (0.5mg) Lane B: GFP transfected 293 cell lysate (0.5mg) Anti-GFP Tag mouse monoclonal antibody at 1/500-1/2000 dilution and 60μg of Immunomagnetic beads Protein G. Primary antibody: Anti-GFP Tag rabbit monoclonal antibody, at 1/1000-1/5000 dilution Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions.
Image 4 Alt TextAequorea victoria GFP Tag Immunoprecipitation(IP) 34491
Image 4 URLSource image

Background Information

DescriptionGreen fluorescent protein (GFP tag) is a ~27 kDa protein consisting of 238 amino acids derived from jellyfish Aequorea Victoria. GFP exhibits bright green fluorescent light when exposed to blue light. It has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms. Anti GFP-tag antibodies are often used in biological research and related fields. GFP tag antibodies are also often been used to study the expression of proteins and the interaction between proteins and proteins.
References
  1. Zhao X, et al. (2013) Several affinity tags commonly used in chromatographic purification. J Anal Methods Chem 2013 581093.
  2. Monti M, et al. (2007) Functional proteomics: Protein-protein interactions in vivo. Ital J Biochem 56 (4): 310-314.
  3. Brothers SP, et al. (2003) Unexpected effects of epitope and chimeric tags on gonadotropin-releasing hormone receptors: Implications for understanding the molecular etiology of hypogonadotropic hypogonadism. J Clin Endocrinol Metab 88 (12): 6107-6112.

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