| Catalog No. | Size | Price (USD) |
| 100393-T02-50 | 50 µL | $147.96 |
| 100393-T02-100 | 100 µL | $251.64 |
| 100393-T02-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-Glypican 3/GPC3 Antibody, Rabbit Polyclonal |
| Validated applications | ICC/IF (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human Glypican 3/GPC3 |
| Immunogen | A synthetic peptide corresponding to the C-terminus of the Human Glypican 3 / GPC3 |
| Preparation | Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Glypican 3 / GPC3, and purified by antigen affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | PBS, pH7.0 with 0.03% Proclin300 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-DGSX Antibody; Anti-GTR2-2 Antibody; Anti-MXR7 Antibody; Anti-OCI-5 Antibody; Anti-OCI5 Antibody; Anti-SDYS Antibody; Anti-SGB Antibody; Anti-SGBS Antibody; Anti-SGBS1 Antibody
Image / Experimental Information
| Image 1 Description | Immunofluorescence staining of Glypican 3 / GPC3 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human Glypican 3 / GPC3 polyclonal antibody (dilution ratio 1:100) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm. |
| Image 1 Alt Text | Human Glypican 3/GPC3 Immunofluorescence(IF) 35326 |
| Image 1 URL | Source image |
Background Information
| Full Name | glypican 3 |
| Description | Glypican-3, also known as Intestinal protein OCI-5, GPC3, and OCI5, is a member of the glypican family. It belongs to the glypican family and is highly expressed in the lung, liver, and kidney. It is a heparan sulfate proteoglycan, which is overexpressed in various neoplasms such as hepatocellular carcinoma, malignant melanoma, and testicular yolk sac tumor, and plays an important role in cell growth and differentiation. GPC3 function is tissue-dependent. In some tissues, GPC3 acts as a tumor suppressor gene, whereas in others, it acts as an oncofetal protein. Studies have shown that GPC3 is a reliable marker for hepatocellular carcinoma. The sensitivity and specificity exceed both alpha-fetoprotein and hepatocyte-paraffin1. GPC3 immunohistochemistry can aid in the differentiation of testicular germ cell tumors, being expressed in all yolk sac tumors but not in seminomas. GPC3 expression has also been identified in some squamous cell carcinomas of the lung and clear cell carcinomas of the ovary. The role of GPC3 in melanomas is still controversial. Thus, Glypican-3 is currently regarded as a tumor marker and potential target for immunotherapy. |
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