This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human GPRC5D. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography, and conjugated with APC under optimum conditions, the unreacted APC was removed.
Source
Monoclonal Mouse IgG2b Clone #12
Purification
Protein A
Formulation
PBS solution containing 0.5% BSA and 0.03%ProClin300
Concentration
5 μL/Test, 0.1 mg/mL
Conjugate
APC
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze !
Image / Experimental Information
Image 1 Description
Flow cytometric analysis of Human GPRC5D expression on CHO-K1 cells.Cells were stained with APC-conjugated anti-Human GPRC5D. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Orphan G-protein-coupled receptor family C group 5 member D (GPRC5D) has seven transmembrane segments forming the core of the receptor, as well as an intracellular C-tail and an extracellular N-terminal domain. GPRC5D is highly and selectively expressed on the surface of CD138+ multiple myeloma (MM) cells, whereas in normal tissues, a much lower expression is limited to plasma cells and certain hard keratinizing tissues. This makes GPRC5D a promising marker for monitoring tumor load and a target antigen for MM-specific T cell-engaging therapies such as bispecific antibodies and CAR-T cells.
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