| Catalog No. | Size | Price (USD) |
| 203891-T38-50 | 50 µL | $147.96 |
| 203891-T38-100 | 100 µL | $251.64 |
| 203891-T38-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-ALKBH5 Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), ICC/IF (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human ALKBH5 |
| Immunogen | E. coli-derived Human ALKBH5 fragment |
| Preparation | Produced in rabbits immunized with E. coli-derived Human ALKBH5 fragment, and purified by antigen affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-ABH5 Antibody; Anti-FLJ20308 Antibody; Anti-OFOXD Antibody; Anti-OFOXD1 Antibody
Image / Experimental Information
| Image 1 Description | Anti-ALKBH5 rabbit polyclonal antibody at 1:500 dilution Lane A: 293 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:44 kDa Observed band size:48 kDa |
| Image 1 Alt Text | Human ALKBH5 Western blot (WB) 34945 |
| Image 1 URL | Source image |
| Image 2 Description | Anti-ALKBH5 rabbit polyclonal antibody at 1:500 dilution Lane A: 293 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:44 kDa Observed band size:48 kDa |
| Image 2 Alt Text | Human ALKBH5 Immunofluorescence(IF) 34946 |
| Image 2 URL | Source image |
| Image 3 Description | Anti-ALKBH5 rabbit polyclonal antibody at 1:500 dilution Lane A: 293 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:44 kDa Observed band size:48 kDa |
| Image 3 Alt Text | Human ALKBH5 Immunoprecipitation(IP) 34947 |
| Image 3 URL | Source image |
Background Information
| Description | AlkB homolog 5 (ALKBH5), an m(6)A demethylase, specifically removes m6A from target mRNAs. ALKBH5, which is most highly expressed in the testes but has low expression in the heart and brain, affects nuclear RNA export and metabolism, gene expression and mouse fertility. Alkbh5 knockout mice were viable but showed compromised spermatogenesis due to significantly altered expression of key genes required for spermatogenic maturation although the increase of overall m6A levels in testes is modest. ALKBH5-mediated m6A erasure in the nuclei of spermatocytes and round spermatids is essential for correct splicing and the production of longer 3'-UTR mRNAs, and failure to do so leads to aberrant splicing and production of shorter transcripts with elevated levels of m6A that are rapidly degraded. ALKBH5 is a prognostic marker for pancreatic cancer. |
| References |
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