| Catalog No. | Size | Price (USD) |
| 11229-MM04-20 | 20 µL | $118.49 |
| 11229-MM04-50 | 50 µL | $222.17 |
| 11229-MM04-100 | 100 µL | $370.28 |
General Information
| Product name | Influenza A H9N2 Hemagglutinin / HA Antibody, Mouse MAb |
| Validated applications | WB (FAQ Protocol) |
| Application notes | IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.) |
| Specificity | H9N2 (A/Hong Kong/1073/99) HA No cross-reactivity in ELISA with H1N1 (A/California/04/2009) HA H1N1 (A/California/07/2009) HA H1N1 (A/Brisbane/59/2007) HA H5N1 (A/Anhui/1/2005)HA H5N1 (A/Viet nam/1194/2004) HA H5N1 (A/Indonesia/5/2005) HA H5N1 (A/bar-headed goose/Qinghai/14/2008) HA H5N1 (A/turkey/Turkey/1/2005) HA H3N2 (A/Brisbane/10/2007) HA H7N7 (A/Netherlands/219/03) HA H2N2 (A/Japan/305/1957) HA Influenza B (B/Florida/04/2006) HA |
| Immunogen | Recombinant H9N2 HA protein (Catalog#11229-V08H) |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Influenza A virus H9N2 Hemagglutinin. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Source | Monoclonal Mouse IgG1 Clone #3F11E10G1 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Background Information
| Full Name | Hemagglutinin |
| Description | The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity. |
| References |
|
title: A Universal Aptamer for Influenza A Viruses: Selection, Recognition, and Infection Inhibition
authors: Meng Wang; Meng-Chan Hao; ...; Zhi-Ling Zhang
journal: ACS pharmacology & translational science
date: 2023-12-29
pmid: pm38230279
cited_by_count: 0
techniques: Recombinant
bioz_score: 95
title: Chicken dendritic cell-targeting nanobodies mediated improved protective effects against H9N2 influenza virus challenge in a homologous sequential immunization study.
authors: Futing Jia; Chao Sun; ...; Chunfeng Wang
journal: Veterinary Microbiology
date: 2023-10-02
pmid: 37729705
doi: 10.1016/j.vetmic.2023.109875
cited_by_count: 9
techniques: Blocking Assay; Centrifugation; Concentration Assay; Construct; Electron Microscopy; Filtration; Incubation; Protease Inhibitor; Protein Purification; Purification; Saline; Staining; Transmission Assay; Western Blot
bioz_score: 95
title: Sphingomyelin-Sequestered Cholesterol Domain Recruits Formin-Binding Protein 17 for Constricting Clathrin-Coated Pits in Influenza Virus Entry.
authors: Bo Tang; En-Ze Sun; ...; Dai-Wen Pang
journal: Journal of virology
date: 2022-04-28
pmid: 35020471
doi: 10.1128/JVI.01813-21
cited_by_count: 7
techniques: Cell Culture; Column Chromatography; Fluorescence; Imaging; Incubation; Inverted Microscopy; Lysis; Microscopy; Purification; Recombinant; Sonication
bioz_score: 95
title: Lipid-specific labeling of enveloped viruses with quantum dots for single-virus tracking
authors: Zhang Li-Juan; Wang Shaobo; ...; Pang Dai-Wen
journal: bioRxiv
date: 2020-01-06
doi: 10.1101/2020.01.06.895805
cited_by_count: 0
techniques: Immunofluorescence
bioz_score: 95
title: Enzyme-induced metallization as a signal amplification strategy for highly sensitive colorimetric detection of avian influenza virus particles.
authors: Chuan-Hua Zhou; Jing-Ya Zhao; ...; Zhi-Ling Zhang
journal: Analytical Chemistry
date: 2015-06-17
pmid: 24475750
cited_by_count: 133
techniques: Magnetic Beads
bioz_score: 95
title: Electrochemical magnetoimmunosensing approach for the sensitive detection of H9N2 avian influenza virus particles.
authors: Chuan-Hua Zhou; Yun Shu; ...; Zhi-Ling Zhang
journal: Chemistry - An Asian Journal
date: 2014-03-21
pmid: 23794357
doi: 10.1002/asia.201300521
cited_by_count: 22
techniques: Magnetic Beads
bioz_score: 95
title: A magnetic bead-based bienzymatic electrochemical immunosensor for determination of H9N2 avian influenza virus
authors: Chuan-Hua Zhou; Yan-Min Long; ...; Zhi-Ling Zhang
journal: Electrochemistry Communications
date: 2013-06-01
doi: 10.1016/j.elecom.2013.03.024
cited_by_count: 42
techniques: Avidin-Biotin Assay; Plasmid Preparation
bioz_score: 95
title: A Simple Point-of-Care Microfluidic Immunomagnetic Fluorescence Assay for Pathogens
authors: Rui-Qiao Zhang; Shu-Lin Liu; ...; Zhi-Ling Zhang
journal: Analytical Chemistry
date: 2013-02-22
doi: 10.1021/ac302903p
cited_by_count: 75
techniques: Virus
bioz_score: 95
