| Catalog No. | Size | Price (USD) |
| 11233-RP01-20 | 20 µL | $88.87 |
| 11233-RP01-100 | 100 µL | $251.64 |
| 11233-RP01-200 | 200 µL | $370.28 |
General Information
| Product name | Human Immunodeficiency Virus type 1 (HIV-1) gp120 / Glycoprotein 120 Antibody, Rabbit PAb |
| Validated applications | WB (FAQ Protocol), ELISA (FAQ Protocol) |
| Application notes | IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.) |
| Specificity | HIV-1 (group M, subtype CRF07_BC) gp120 |
| Immunogen | Recombinant HIV-1 GP120 protein (Catalog#11233-V08H) |
| Preparation | Produced in rabbits immunized with purified, recombinant HIV-1 gp120 (rv GP120; Catalog#11233-V08H;Ala29-Glu502; AFM77980.1). Total IgG was purified by Protein A affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Background Information
| Description | The HIV-1 gp120 envelope protein, a glycoprotein that is part of the outer layer of the virus, is an essential component in the multi-tiered viral entry process. It presents itself as viral membrane spikes consisting of 3 molecules of gp120 linked together and anchored to the membrane by gp41 protein. Gp120 is essential for viral infection as it facilitates HIV entry into the host cell and this is its best-known and most researched role in HIV infection. However, it is becoming increasingly evident that gp120 might also be facilitating viral persistence and continuing HIV infection by influencing the T cell immune response to the virus. The surface protein gp120 attaches the virus to the host lymphoid cell by binding to the primary receptor CD4. Gp120 binding to its receptor CD4 and co-receptor, CXCR4 or CCR5 is required for fusion of viral and cellular membranes. Several mechanisms might be involved in this process of which gp120 binding to the CD4 receptor of T cells is the best known and most important interaction as it facilitates viral entry into the CD4+ cells and their depletion, a hallmark of the HIV infection. Gp120 is shed from the viral membrane and accumulates in lymphoid tissues in significant amounts. Despite the overall genetic heterogeneity of the gp120 glycoprotein, the conserved CD4 binding site provides an attractive antiviral target. Interaction between gp120 and ITGA4/ITGB7 would allow the virus to enter GALT early in the infection, infecting and killing most of GALT's resting CD4+ T-cells. This T-cell depletion is believed to be the major insult to the host immune system leading to AIDS. |
| References |
|
title: In Vitro Selection and Characterization of HIV-1 Variants with Increased Resistance to LP-40, Enfuvirtide-Based Lipopeptide Inhibitor
authors: Yue Hu; Wenjiang Yu; ...; Maria Grazia Cifone
journal: International Journal of Molecular Sciences
date: 2022-06-14
pmid: 35743078
doi: 10.3390/ijms23126638
pmcid: 9223764
cited_by_count: 2
techniques: Bicinchoninic Acid Protein Assay; Blocking Assay; Concentration Assay; Expressing; Incubation; Lysis; Mutagenesis; Protein Concentration; SDS Page; Transfection; Western Blot
bioz_score: 90
title: Cell membrane-anchored anti-HIV single-chain antibodies and bifunctional inhibitors targeting the gp41 fusion protein: new strategies for HIV gene therapy
authors: Yue Chen; Hongliang Jin; ...; Yuxian He
journal: Emerging microbes & infections
date: 2021-12-21
pmid: 34821542
doi: 10.1080/22221751.2021.2011616
pmcid: 8735881
cited_by_count: 10
techniques: Imaging; Incubation; Infection; SDS Page
bioz_score: 90
title: Generation of HIV-resistant cells with a single-domain antibody: implications for HIV-1 gene therapy
authors: Hongliang Jin; Xiaoran Tang; ...; Yuxian He
journal: Cellular & molecular immunology
date: 2021-01-18
pmid: 33462383
doi: 10.1038/s41423-020-00627-y
pmcid: 7812570
cited_by_count: 11
techniques: Derivative Assay; Expressing; Imaging; Incubation; SDS Page; Western Blot
bioz_score: 90
title: Structural and Functional Characterization of the Secondary Mutation N126K Selected by Various HIV-1 Fusion Inhibitors
authors: Danwei Yu; Yang Su; ...; Yuxian He
journal: Viruses
date: 2020-03-18
pmid: 32197300
doi: 10.3390/v12030326
pmcid: 7150849
cited_by_count: 2
techniques: Blocking Assay; Control; Enzyme-linked Immunosorbent Assay; Expressing; Flow Cytometry; Imaging; Incubation; Lysis; Membrane; Mutagenesis; SDS Page; Saline; Transfection; Western Blot
bioz_score: 90
title: Conserved Residue Asn-145 in the C-Terminal Heptad Repeat Region of HIV-1 gp41 is Critical for Viral Fusion and Regulates the Antiviral Activity of Fusion Inhibitors
authors: Xiuzhu Geng; Zixuan Liu; ...; Yuxian He
journal: Viruses
date: 2019-07-03
pmid: 31277353
doi: 10.3390/v11070609
pmcid: 6669600
cited_by_count: 6
techniques: Blocking Assay; Expressing; Imaging; Incubation; Mutagenesis; Transfection; Western Blot
bioz_score: 90
