| Catalog No. | Size | Price (USD) |
| 11277-RP02-50 | 50 µL | $147.96 |
| 11277-RP02-100 | 100 µL | $251.64 |
| 11277-RP02-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-GPA33/A33 Antibody, Rabbit Polyclonal |
| Validated applications | ELISA (FAQ Protocol), IHC-P (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human GPA33/A33 |
| Immunogen | Recombinant Human GPA33 protein (Catalog#11277-H08H) |
| Preparation | Produced in rabbits immunized with purified, recombinant Human GPA33 (rh GPA33; Catalog#11277-H08H; Q99795-1; Met 1-Val 235). GPA33 specific IgG was purified by Human GPA33 affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-A33 Antibody
Image / Experimental Information
| Image 1 Description | Immunochemical staining of human GPA33 in human rectal cancer with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium. |
| Image 1 Alt Text | Human GPA33 Immunohistochemistry(IHC) 4097 |
| Image 1 URL | Source image |
| Image 2 Description | Immunochemical staining of human GPA33 in human rectal cancer with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium. |
| Image 2 Alt Text | Human GPA33 Immunohistochemistry(IHC) 4098 |
| Image 2 URL | Source image |
| Image 3 Description | Immunochemical staining of human GPA33 in human rectal cancer with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium. |
| Image 3 Alt Text | Human GPA33 Immunohistochemistry(IHC) 4100 |
| Image 3 URL | Source image |
| Image 4 Description | Immunochemical staining of human GPA33 in human rectal cancer with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium. |
| Image 4 Alt Text | Human GPA33 Immunohistochemistry(IHC) 4102 |
| Image 4 URL | Source image |
Background Information
| Full Name | glycoprotein A33 (transmembrane) |
| Description | Cell surface A33 antigen, also known as glycoprotein A33, is a single-pass type I membrane protein that is expressed in the normal gastrointestinal epithelium and 95% of colon cancers. GPA33 contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. Intracellular traffic and recycling to the cell surface appear to play a major role in GPA33 function and to have an influence on its surface density superseding translational regulation. GPA33 has become a promising target of immunologic therapy strategies, but its biologic function and potential role in tumorigenesis are unknown. EpCAM protein and GPA33 mRNA expressions are specific and sensitive markers of Barrett's metaplasia (BM). GPA33 may also play a role in cell-cell recognition and signaling. |
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