Recombinant Anti-AARSD1 Antibody, Rabbit Monoclonal sinobiological 14280-R012

$171.00
In stock
SKU
14280-R012
Catalog No.SizePrice (USD)
14280-R012-5050 µL$177.58
14280-R012-100100 µL$340.51

General Information

Product nameRecombinant Anti-AARSD1 Antibody, Rabbit Monoclonal
Validated applicationsWB (FAQ Protocol), ELISA (FAQ Protocol), ICC/IF (FAQ Protocol)
Species reactivityReacts with: Human
SpecificityHuman AARSD1
ImmunogenRecombinant Human AARSD1 Protein (Catalog#14280-H07E)
PreparationThis antibody was obtained from a rabbit immunized with purified, recombinant Human AARSD1 (Catalog #14280-H07E; Q9BTE6-2; Thr176-Glu525).
SourceMonoclonal Rabbit IgG Clone #012
PurificationProtein A
Formulation0.2 μm filtered solution in PBS
ConjugateUnconjugated
FormLiquid
ShippingThis antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
StorageThis antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Synonyms: Anti-AlaXp Antibody; Anti-MGC2744 Antibody

Image / Experimental Information

Image 1 DescriptionImmunofluorescence staining of Human AARSD1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human AARSD1 monoclonal antibody (1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Image 1 Alt TextHuman AARSD1 Immunohistochemistry(IHC) 3020
Image 1 URLSource image
Image 2 DescriptionImmunofluorescence staining of Human AARSD1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human AARSD1 monoclonal antibody (1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Image 2 Alt TextHuman AARSD1 Western blot (WB) 6987
Image 2 URLSource image

Background Information

Full Namealanyl-tRNA synthetase domain containing 1
DescriptionAARSD1 belongs to the class-II aminoacyl-tRNA synthetase family, Alax-L subfamily. AARSD1 binds 1 zinc ion per subunit functions in trans to edit the amino acid moiety from incorrectly charged tRNA(Ala). Four transcript variants have been described for AARSD1: NM_25267.3, NM_113642.2, NM_1142653.1 and NM_1142654.1. It has been determined that the latter two variants represent a distinct upstream locus, which is now represented by GeneID:1885848 (PTGES3L), while the former two variants represent readthrough transcripts between PTGES3L and this locus (AARSD1). The readthrough locus (PTGES3L-AARSD1) is now represented by GeneID:188585.
References
  1. Tatham MH, et al. (2011) Comparative proteomic analysis identifies a role for SUMO in protein quality control. Oncogene. 19(17):2120-8.
  2. Kim W, et al. (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell. 44(2):325-40.
  3. Udeshi ND, et al. (2012) Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition. Mol Cell Proteomics. 11(5):148-59.

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