| Catalog No. | Size | Price (USD) |
| 50337-R001-50 | 50 µL | $177.58 |
| 50337-R001-100 | 100 µL | $340.51 |
General Information
| Product name | Recombinant Anti-Angiotensinogen Antibody, Rabbit Monoclonal |
| Validated applications | WB (FAQ Protocol) |
| Species reactivity | Reacts with: Mouse |
| Specificity | Mouse Angiotensinogen |
| Immunogen | Recombinant Mouse Angiotensinogen/AGT Protein (Catalog#50337-M08H) |
| Preparation | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Angiotensinogen/AGT (rM Angiotensinogen/AGT; Catalog#50337-M08H; P11859; Met1-Val477). |
| Source | Monoclonal Rabbit IgG Clone #001 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS with 10% Trehalose, pH7.0 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-Agt Antibody; Anti-AngI Antibody; Anti-AngII Antibody; Anti-Aogen Antibody; Anti-Serpina8 Antibody
Image / Experimental Information
| Image 1 Description | Anti-AGT rabbit monoclonal antibody at 1:1000 dilution Lane A: Mouse spleen tissue lysate Lane B: Mouse liver tissue lysate Lane C: Mouse kidney tissue lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:51 kDa |
| Image 1 Alt Text | Mouse Angiotensinogen Western blot (WB) 22253 |
| Image 1 URL | Source image |
Background Information
| Full Name | angiotensinogen (serpin peptidase inhibitor, clade A, member 8) |
| Description | Angiotensinogen, also known as AGT and SerpinA8, is a member of the serpin family. It is an α-2-globulin that is produced constitutively and released into the circulation mainly by the liver. Angiotensinogen is a essential component of the renin-angiotensin system (RAS) and a potent regulator of blood pressure. Angiotensinogen can be schematically considered to consist of a combination of an angiotensin I (Ang I) function, located at the N-terminal end, and the presence of a serpin (serine protease inhibitor) structure at the opposite end. Angiotensinogen is cleaved into three chains: Angiotensin-1 (Ang I), Angiotensin-2 (Ang II), and Angiotensin-3 (Ang III). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2. Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3, angiotensin-4. Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2. Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system. Defects in AGT are associated with susceptibility to essential hypertension and renal tubular dysgenesis (RTD). Several serpins (antithrombin, maspin, pigment epithelial-derived factor, and kallistatin) have been recently shown to exert an antiangiogenic activity, suggesting a common mechanism of endothelial cell proliferation and migration. Angiotensinogen/AGT and its renin-cleaved product, des(Ang I)AGT, are also angiogenesis inhibitors, both in vitro and in vivo at concentrations within the range of those observed in plasma. The Angiotensinogen products, that is angiotensin II and possibly angiotensin II-related products, have been found to act locally in modulating adipose tissue growth in an autocrine/paracrine manner. The transient or chronic overexpression of angiotensinogen in adipose tissue favors lipogenesis in adipocytes and leads to a 'vicious' circle whereby adipose tissue development is further increased. |
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