| Catalog No. | Size | Price (USD) |
| 101273-T40-20 | 20 µL | $88.87 |
| 101273-T40-50 | 50 µL | $147.96 |
| 101273-T40-100 | 100 µL | $251.64 |
| 101273-T40-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-Actin Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), IHC-P (FAQ Protocol) |
| Species reactivity | Reacts with: Human, Mouse, Rat |
| Specificity | Human, Mouse, Rat |
| Immunogen | A synthetic peptide corresponding to the N-terminus of the Human Actin |
| Preparation | Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human Actin, and purified by antigen affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-ACTA Antibody; Anti-ASMA Antibody; Anti-CFTD Antibody; Anti-CFTD1 Antibody; Anti-CFTDM Antibody; Anti-CMYP2A Antibody; Anti-CMYP2B Antibody; Anti-CMYP2C Antibody; Anti-MPFD Antibody; Anti-NEM1 Antibody; Anti-NEM2 Antibody; Anti-NEM3 Antibody; Anti-SHPM Antibody
Image / Experimental Information
| Image 1 Description | Anti-ACTA1 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:42 kDa Observed band size:45 kDa |
| Image 1 Alt Text | Human Actin Western blot (WB) 19393 |
| Image 1 URL | Source image |
| Image 2 Description | Anti-ACTA1 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:42 kDa Observed band size:45 kDa |
| Image 2 Alt Text | Human Actin Immunohistochemistry(IHC) 19394 |
| Image 2 URL | Source image |
| Image 3 Description | Anti-ACTA1 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:42 kDa Observed band size:45 kDa |
| Image 3 Alt Text | Human Actin Immunohistochemistry(IHC) 19395 |
| Image 3 URL | Source image |
| Image 4 Description | Anti-ACTA1 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:42 kDa Observed band size:45 kDa |
| Image 4 Alt Text | Human Actin Immunohistochemistry(IHC) 19396 |
| Image 4 URL | Source image |
| Image 5 Description | Anti-ACTA1 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:42 kDa Observed band size:45 kDa |
| Image 5 Alt Text | Human Actin Immunohistochemistry(IHC) 19397 |
| Image 5 URL | Source image |
Background Information
| Full Name | actin, alpha 1, skeletal muscle |
| Description | Nemaline myopathy (NM) is one of the most common congenital nondystrophic myopathies and is characterized by muscle weakness, often from birth. Mutations in ACTA1 are a frequent cause of NM (ie, NEM3). ACTA1 encodes alpha-actin 1, the main constituent of the sarcomeric thin filament. The mechanisms by which mutations in ACTA1 contribute to muscle weakness in NEM3 are incompletely understood. Mutations in skeletal muscle α-actin 1-encoding gene (ACTA1) cause autosomal dominant or recessive myopathies with marked clinical and pathological heterogeneity. Patients typically develop generalized or limb-girdle pattern of weakness, that clinically severe ACTA1-related myopathy can present with muscle morphological findings suggestive of cytoplasmic body myopathy in the absence of definite nemaline rods. The Asn94Lys mutation in skeletal muscle sarcomeric α-actin may be linked to this histological appearance. |
title: Ultrathin BSA-Stabilized Black Phosphorous Nanoreactor Boosts Mild-Temperature Photothermal Therapy Through Modulation of Cellular Self-Defense Fate.
authors: Guoping Jia; Qinghe Wu; ...; Chunfu Zhang
journal: Advanced Healthcare Materials
date: 2024-12-17
pmid: 39225409
doi: 10.1002/adhm.202402079
cited_by_count: 6
techniques: CCK-8 Assay; Cell Culture; Purification
bioz_score: 92
title: Chlorogenic acid inhibits the proliferation of human lung cancer A549 cell lines by targeting annexin A2 in vitro and in vivo.
authors: Lei Wang; Hongwu Du; Peng Chen
journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
date: 2021-02-18
pmid: 32882585
doi: 10.1016/j.biopha.2020.110673
cited_by_count: 80
techniques: Enzyme-linked Immunosorbent Assay; Imaging; Incubation; Membrane; SDS Page; Western Blot
bioz_score: 92
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