| Catalog No. | Size | Price (USD) |
| 12177-T52-50 | 50 µL | $147.96 |
| 12177-T52-100 | 100 µL | $251.64 |
| 12177-T52-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-Alpha-fetoprotein Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), ELISA (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human Alpha-fetoprotein |
| Immunogen | Recombinant Human AFP / alpha-fetoprotein protein (Catalog#12177-H08H) |
| Preparation | Produced in rabbits immunized with purified, recombinant Human AFP / alpha-fetoprotein (rh AFP / alpha-fetoprotein; Catalog#12177-H08H; P02771; Met1-Val609). AFP / alpha-fetoprotein specific IgG was purified by Human AFP / alpha-fetoprotein affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-AFPD Antibody; Anti-FETA Antibody; Anti-HPAFP Antibody
Image / Experimental Information
| Image 1 Description | AFP was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 2 µL anti-AFP rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-AFP rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 68 kDa Observed band size: 68 kDa |
| Image 1 Alt Text | Human Alpha-fetoprotein/AFP Immunoprecipitation(IP) 6038 |
| Image 1 URL | Source image |
| Image 2 Description | AFP was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 2 µL anti-AFP rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-AFP rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 68 kDa Observed band size: 68 kDa |
| Image 2 Alt Text | Human Alpha-fetoprotein/AFP Western blot (WB) 6660 |
| Image 2 URL | Source image |
Background Information
| Full Name | alpha-fetoprotein |
| Description | Alpha-fetoprotein (AFP) is classified as a member of the albuminoid gene superfamily consisting of albumin, AFP, vitamin D (Gc) protein, and alpha-albumin. AFP is a glycoprotein of 591 amino acids and a carbohydrate moiety. AFP is one of the several embryo-specific proteins and is a dominant serum protein as early in human embryonic life as one month, when albumin and transferrin are present in relatively small amounts. It is first synthesized in the human by the yolk sac and liver(1-2 months) and subsequently predominantly in the liver. A small amount of AFP is produced by the GI tract of the human conceptus. It has been proved that AFP may reappear in the serum in elevated amounts in adult life in association with normal restorative processes and with malignant growth. Alpha-fetoprotein (AFP) is a specific marker for hepatocellular carcinoma (HCC), teratoblastomas, and neural tube defect (NTD). |
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| References |
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title: Sensitive fluorescence immunoassay of alpha-fetoprotein through copper ions modulated growth of quantum dots in-situ
authors: Yi-Wei Wang; Liye Chen; ...; Hongbo Song
journal: Sensors and Actuators B: Chemical
date: 2017-08-01
doi: 10.1016/j.snb.2017.03.036
cited_by_count: 33
techniques: Binding Assay; Enzyme-linked Immunosorbent Assay; Fluorescence
bioz_score: 90
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