WB (FAQ Protocol), IHC-P (FAQ Protocol), IP (FAQ Protocol)
Species reactivity
Reacts with: Human
Specificity
Human AP2A2
Immunogen
E. coli-derived Human AP2A2 fragment
Preparation
Produced in rabbits immunized with E. coli-derived Human AP2A2 fragment, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
PBS, pH7.0 with 0.03% Proclin300
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.
Anti-AP2A2 rabbit polyclonal antibody at 1:500 dilution Lane A: RT-4 Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:104 kDa Observed band size:104 kDa
Anti-AP2A2 rabbit polyclonal antibody at 1:500 dilution Lane A: RT-4 Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:104 kDa Observed band size:104 kDa
Anti-AP2A2 rabbit polyclonal antibody at 1:500 dilution Lane A: RT-4 Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:104 kDa Observed band size:104 kDa
Anti-AP2A2 rabbit polyclonal antibody at 1:500 dilution Lane A: RT-4 Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:104 kDa Observed band size:104 kDa
AP-2 is a stable protein complex comprising 4 domains, which are termed alpha, beta, mu, and sigma. AP2A1 and AP2A2 are homologous human genes that encode the alpha subunit of the AP-2 complex. The AP2A2 transcript is expressed in a broad range of CNS cell lineages. At the tissue level, both AP2A1 and AP2A2 are highly expressed in human cerebellum, but AP2A2 is expressed at lower levels in the cerebral cortex. AP2A2 (Adaptor Related Protein Complex 2 Subunit Alpha 2, also known as HIP9; HYPJ; ADTAB; HIP-9; CLAPA2) is located on human chromosome 11p15.5. The AP2A2 protein is ubiquitously expressed in spleen, brain and other tissues. Structurally, the encoded AP2A2 protein is reported to be 103960 Da in mass.
References
Nelson PT, et al. (2020) The muc6/ap2a2 locus and its relevance to alzheimer's disease: A review. J Neuropathol Exp Neurol 79 (6): 568-584.