| Catalog No. | Size | Price (USD) |
| 11942-T62-50 | 50 µL | $147.96 |
| 11942-T62-100 | 100 µL | $251.64 |
| 11942-T62-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-ANP32A Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), ELISA (FAQ Protocol), IHC-P (FAQ Protocol), ICC/IF (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human ANP32A |
| Immunogen | Recombinant Human ANP32A / PHAP1 Protein (Catalog#11942-H20E) |
| Preparation | Produced in rabbits immunized with purified, recombinant Human ANP32A / PHAP1 (rh ANP32A / PHAP1; Catalog#11942-H20E; NP_006296.1; Glu2-Lys238). ANP32A / PHAP1 specific IgG was purified by Human ANP32A / PHAP1 affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-C15orf1 Antibody; Anti-HPPCn Antibody; Anti-I1PP2A Antibody; Anti-LANP Antibody; Anti-MAPM Antibody; Anti-mapmodulin Antibody; Anti-PHAP1 Antibody; Anti-PHAPI Antibody; Anti-PP32 Antibody
Image / Experimental Information
| Image 1 Description | Anti-ANP32A rabbit polyclonal antibody at 1:500 dilution Lane A: HEK293 Whole Cell Lysate Lane B: Raji Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:28 kDa |
| Image 1 Alt Text | Human ANP32A / PHAP1 Western blot (WB) 15731 |
| Image 1 URL | Source image |
| Image 2 Description | Anti-ANP32A rabbit polyclonal antibody at 1:500 dilution Lane A: HEK293 Whole Cell Lysate Lane B: Raji Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:28 kDa |
| Image 2 Alt Text | Human ANP32A / PHAP1 Immunohistochemistry(IHC) 15732 |
| Image 2 URL | Source image |
| Image 3 Description | Anti-ANP32A rabbit polyclonal antibody at 1:500 dilution Lane A: HEK293 Whole Cell Lysate Lane B: Raji Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:28 kDa |
| Image 3 Alt Text | Human ANP32A / PHAP1 Immunohistochemistry(IHC) 15733 |
| Image 3 URL | Source image |
| Image 4 Description | Anti-ANP32A rabbit polyclonal antibody at 1:500 dilution Lane A: HEK293 Whole Cell Lysate Lane B: Raji Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:28 kDa |
| Image 4 Alt Text | Human ANP32A / PHAP1 Immunofluorescence(IF) 15734 |
| Image 4 URL | Source image |
| Image 5 Description | Anti-ANP32A rabbit polyclonal antibody at 1:500 dilution Lane A: HEK293 Whole Cell Lysate Lane B: Raji Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:28 kDa |
| Image 5 Alt Text | Human ANP32A / PHAP1 Immunoprecipitation(IP) 15735 |
| Image 5 URL | Source image |
Background Information
| Full Name | acidic (leucine-rich) nuclear phosphoprotein 32 family, member A |
| Description | Acidic leucine-rich nuclear phosphoprotein 32 family member A, also known as acidic nuclear phosphoprotein pp32, Leucine-rich acidic nuclear protein, Mapmodulin, Potent heat-stable protein phosphatase 2A inhibitor I1PP2A, Putative HLA-DR-associated protein I, PHAPI and ANP32A, is a nucleus, cytoplasm and endoplasmic reticulum. ANP32A / LANP is expressed in all tissues tested. It is highly expressed in kidney and skeletal muscle, moderate levels of expression is in brain, placenta and pancreas. ANP32A / LANP is weakly expressed in lung. It is found in all regions of the brain examined (amygdala, caudate nucleus, corpus callosum, hippocampus and thalamus), with highest levels in amygdala. ANP32A / LANP is a component of the SET complex, which also contains SET, APEX1, HMGB2 and NME1. It directly interacts with SET. ANP32A / LANP also interacts with ATXN1/SCA1. ANP32A / LANP is implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. ANP32A / LANP plays a role in E4F1-mediated transcriptional repression. |
| References |
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