| Catalog No. | Size | Price (USD) |
| 100801-T46-50 | 50 µL | $147.96 |
| 100801-T46-100 | 100 µL | $251.64 |
| 100801-T46-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-APE1/APEX1 Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), IHC-P (FAQ Protocol), ICC/IF (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human APE1/APEX1 |
| Immunogen | A synthetic peptide corresponding to the N-terminus of the Human APEX1 / AP / APEx / Ref-1 |
| Preparation | Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human APEX1 / AP / APEx / Ref-1, and purified by antigen affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-APE Antibody; Anti-APE-1 Antibody; Anti-APE1 Antibody; Anti-APEN Antibody; Anti-APEX Antibody; Anti-APX Antibody; Anti-HAP1 Antibody; Anti-REF-1 Antibody; Anti-REF1 Antibody
Image / Experimental Information
| Image 1 Description | Immunofluorescence staining of APEX1 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human APEX1 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (green)Positive staining was localized to nucleus. |
| Image 1 Alt Text | Human APEX1/APE1/Ref-1 Immunofluorescence(IF) 3085 |
| Image 1 URL | Source image |
| Image 2 Description | Immunofluorescence staining of APEX1 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human APEX1 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (green)Positive staining was localized to nucleus. |
| Image 2 Alt Text | Human APEX1/APE1/Ref-1 Immunohistochemistry(IHC) 4696 |
| Image 2 URL | Source image |
| Image 3 Description | Immunofluorescence staining of APEX1 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human APEX1 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (green)Positive staining was localized to nucleus. |
| Image 3 Alt Text | Human APEX1/APE1/Ref-1 Immunohistochemistry(IHC) 4697 |
| Image 3 URL | Source image |
| Image 4 Description | Immunofluorescence staining of APEX1 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human APEX1 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (green)Positive staining was localized to nucleus. |
| Image 4 Alt Text | Human APEX1/APE1/Ref-1 Immunoprecipitation(IP) 5986 |
| Image 4 URL | Source image |
| Image 5 Description | Immunofluorescence staining of APEX1 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human APEX1 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (green)Positive staining was localized to nucleus. |
| Image 5 Alt Text | Human APEX1/APE1/Ref-1 Western blot (WB) 6521 |
| Image 5 URL | Source image |
Background Information
| Full Name | APEX nuclease (multifunctional DNA repair enzyme) 1 |
| Description | The enzyme is known to be a redox factor (Ref-1) stimulating DNA binding activity of AP-1 binding proteins such as Fos and Jun as well as a multifunctional DNA repair enzyme having 5' AP endonuclease, DNA 3' repair diesterase, 3'-5' exonuclease and DNA 3'-phosphatase activities.Although Apex mRNA was expressed ubiquitously, the levels varied significantly, suggesting organ- or tissue-specific expression of the Apex gene. The highest level was observed in the testis, relatively high levels in the thymus, spleen, kidney and brain, and the lowest level in the liver in rats. However, the present results suggested that APEX/Ref-1 gene product can interact with AP-1 binding proteins in brain, especially in the hippocampal formation, to regulate some brain functions by redox-activation. |
| References |
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