| Catalog No. | Size | Price (USD) |
| 100836-T42-50 | 50 µL | $147.96 |
| 100836-T42-100 | 100 µL | $251.64 |
| 100836-T42-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-ARIH2 Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), IHC-P (FAQ Protocol), ICC/IF (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human Mouse (Species predicted to react based on 100% sequence homology) |
| Immunogen | A synthetic peptide corresponding to the C-terminus of the Human ARIH2 |
| Preparation | Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human ARIH2, and purified by antigen affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-ARI2 Antibody; Anti-HT005 Antibody; Anti-TRIAD1 Antibody
Image / Experimental Information
| Image 1 Description | Immunofluorescence staining of ARIH2 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human ARIH2 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (green)Positive staining was localized to nucleus. |
| Image 1 Alt Text | Human ARIH2/TRIAD1 Immunofluorescence(IF) 2737 |
| Image 1 URL | Source image |
| Image 2 Description | Immunofluorescence staining of ARIH2 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human ARIH2 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (green)Positive staining was localized to nucleus. |
| Image 2 Alt Text | Human ARIH2/TRIAD1 Immunohistochemistry(IHC) 4689 |
| Image 2 URL | Source image |
| Image 3 Description | Immunofluorescence staining of ARIH2 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human ARIH2 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (green)Positive staining was localized to nucleus. |
| Image 3 Alt Text | Human ARIH2/TRIAD1 Western blot (WB) 6526 |
| Image 3 URL | Source image |
Background Information
| Full Name | ariadne RBR E3 ubiquitin protein ligase 2 |
| Description | ARIH2 encodes the Triad1 protein, an anti-proliferative E3 ubiquitin ligase. In the current study, we investigate the role of Triad1 in leukemogenesis induced by an MLL1 fusion protein (Mll-Ell). we investigate the role of Triad1 in leukemogenesis induced by an MLL1 fusion protein (Mll-Ell), relevant Triad1 substrates may indicate novel therapeutic targets in this disease. E3 ligase, Ariadne homolog 2 (ARIH2), as a posttranslational negative regulator of NLRP3 inflammasome activity in macrophages. ARIH2 interacted with NLRP3 via its NACHT domain (aa 220-575) in the NLRP3 inflammasome complex. ARIH2 overexpression promoted NLRP3 ubiquitination and inhibited NLRP3 inflammasome activation. Reveal a novel mechanism of ubiquitination-dependent negative regulation of the NLRP3 inflammasome by ARIH2 and highlight ARIH2 as a potential therapeutic target for inflammatory diseases. |
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