| Catalog No. | Size | Price (USD) |
| 14382-R002-50 | 50 µL | $177.58 |
| 14382-R002-100 | 100 µL | $340.51 |
General Information
| Product name | Recombinant Anti-ASF1B Antibody, Rabbit Monoclonal |
| Validated applications | IHC-P (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human ASF1B |
| Immunogen | Recombinant Human ASF1B Protein (Catalog#14382-H08B) |
| Preparation | This antibody was obtained from a rabbit immunized with purified, recombinant Human ASF1B (rh ASF1B; Catalog#14382-H08B; Q9NVP2; Met1-Ile202). |
| Source | Monoclonal Rabbit IgG Clone #002 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-CIA-II Antibody; Anti-FLJ10604 Antibody
Image / Experimental Information
| Image 1 Description | Immunochemical staining of human ASF1B in human testis with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). |
| Image 1 Alt Text | Human ASF1B Immunohistochemistry(IHC) 4685 |
| Image 1 URL | Source image |
| Image 2 Description | Immunochemical staining of human ASF1B in human testis with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). |
| Image 2 Alt Text | Human ASF1B Immunohistochemistry(IHC) 4686 |
| Image 2 URL | Source image |
Background Information
| Full Name | anti-silencing function 1B histone chaperone |
| Description | The histone chaperone anti-silencing factor 1a (ASF1a) interacts with MDC1 and is recruited to sites of DSBs to facilitate the interaction of phospho-ATM with MDC1 and phosphorylation of MDC1, which are required for the recruitment of RNF8/RNF168 histone ubiquitin ligases. Thus, ASF1a deficiency reduces histone ubiquitination at DSBs, decreasing the recruitment of 53BP1, and decreases NHEJ, rendering cells more sensitive to DSBs. This role of ASF1a in DSB repair cannot be provided by the closely related ASF1b and does not require its histone chaperone activity. Homozygous deletion of ASF1A is seen in 10%-15% of certain cancers, suggesting that loss of NHEJ may be selected in some malignancies and that the deletion can be used as a molecular biomarker for cancers susceptible to radiotherapy or to DSB-inducing chemotherapy. Anti-silencing function 1 (ASF1) is a histone H3-H4 chaperone involved in DNA replication and repair, and transcriptional regulation. Here, we identify ASF1B, the mammalian paralog to ASF1, as a proliferation-inducing histone chaperone in human β-cells. Overexpression of ASF1B led to distinct transcriptional signatures consistent with increased cellular proliferation and reduced cellular death. |
