| Catalog No. | Size | Price (USD) |
| 104727-T40-50 | 50 µL | $147.96 |
| 104727-T40-100 | 100 µL | $251.64 |
| 104727-T40-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-ATP5B Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), IHC-P (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human ATP5B |
| Immunogen | A synthetic peptide corresponding to the center region of the Human ATP5B |
| Preparation | Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human ATP5B, and purified by antigen affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-ATP5B Antibody; Anti-ATPMB Antibody; Anti-ATPSB Antibody; Anti-HEL-S-271 Antibody; Anti-HUMOP2 Antibody
Image / Experimental Information
| Image 1 Description | Anti-ATP5B rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: Mouse heart tissue lysate Lane D: Rat heart tissue lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:56 kDa Observed band size:54 kDa (We are unsure as to the identity of these extra bands.) |
| Image 1 Alt Text | Human ATP5B Western blot (WB) 20124 |
| Image 1 URL | Source image |
| Image 2 Description | Anti-ATP5B rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: Mouse heart tissue lysate Lane D: Rat heart tissue lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:56 kDa Observed band size:54 kDa (We are unsure as to the identity of these extra bands.) |
| Image 2 Alt Text | Human ATP5B Immunohistochemistry(IHC) 20125 |
| Image 2 URL | Source image |
| Image 3 Description | Anti-ATP5B rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: Mouse heart tissue lysate Lane D: Rat heart tissue lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:56 kDa Observed band size:54 kDa (We are unsure as to the identity of these extra bands.) |
| Image 3 Alt Text | Human ATP5B Immunohistochemistry(IHC) 20126 |
| Image 3 URL | Source image |
Background Information
| Full Name | ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide |
| Description | Congenital obstructive nephropathy is the primary cause of chronic renal failure in children. Disorders of mitochondrial energy metabolism may be a primary factor underlying tubular cell apoptosis in hydronephrosis. The beta-F1-ATPase (ATP5B) and electron transfer flavoprotein beta subunit (ETFB) metabolic markers are involved in mitochondrial energy metabolism in other diseases. ATP5B and ETFB were associated with worsening renal injury. ATP5B and ETFB may be novel markers in hydronephrosis. |
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