| Catalog No. | Size | Price (USD) |
| 11188-MM06-1 | 1 mL | $1,377.43 |
| 11188-MM06-20 | 20 µL | $88.87 |
| 11188-MM06-50 | 50 µL | $147.96 |
| 11188-MM06-100 | 100 µL | $251.64 |
| 11188-MM06-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-B7-H3 Antibody, Mouse Monoclonal |
| Validated applications | ELISA (FAQ Protocol), ELISA(Det) (FAQ Protocol), FCM (FAQ Protocol), ICC/IF (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human B7-H3 No cross-reactivity in ELISA with Human cell lysate (293 cell line) |
| Immunogen | Recombinant Human B7-H3 / CD276 protein (Catalog#11188-H08H) |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human B7-H3 / CD276 (rh B7-H3 / CD276; Catalog#11188-H08H; Q5ZPR3-1; Met 1-Thr 461). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Source | Monoclonal Mouse IgG2b Clone #06 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-B7-H3 Antibody; Anti-B7H3 Antibody; Anti-B7RP-2 Antibody; Anti-PSEC0249 Antibody; Anti-UNQ309/PRO352 Antibody
Image / Experimental Information
| Image 1 Description | Immunofluorescence staining of Human B7H3 in PC3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human B7H3 monoclonal antibody (1:100) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane. |
| Image 1 Alt Text | Human B7-H3/CD276 Immunofluorescence(IF) 3004 |
| Image 1 URL | Source image |
| Image 2 Description | Immunofluorescence staining of Human B7H3 in PC3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human B7H3 monoclonal antibody (1:100) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane. |
| Image 2 Alt Text | Human B7-H3/CD276 Flow Cytometry (FC) 5338 |
| Image 2 URL | Source image |
Background Information
| Full Name | CD276 molecule |
| Description | B7-H3 is a member of the B7 family of immune regulatory ligands that is thought to attenuate peripheral immune responses through co-inhibition. It plays an important role in adaptive immune responses, and was shown to either promote or inhibit T-cell responses in various experimental systems. B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is a novel protein structurally related to the B7 family of ligands by the presence of a single set of immunoglobulin-V-like and immunoglobulin-C-like (VC) domains. Previous studies have correlated its overexpression with poor prognosis and decreased tumor-infiltrating lymphocytes in various carcinomas including uterine endometrioid carcinomas, and mounting evidence supports an immuno-inhibitory role in ovarian cancer prognosis. Recently, B7-H3 expression has been reported in several human cancers indicating an additional function of B7-H3 as a regulator of antitumor immunity. |
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| References |
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title: Improving Targeted Delivery and Antitumor Efficacy of TRAIL through Fusion with a B7H3-Antagonistic Affibody.
authors: Xiaomeng Lu; Xinyu Zhan; ...; Dianlong Jia
journal: Molecular Pharmaceutics
date: 2024-12-02
pmid: 39620978
doi: 10.1021/acs.molpharmaceut.4c00891
cited_by_count: 0
techniques: Expressing; Fluorescence; Injection; Microscopy; Optical Imaging; Staining
bioz_score: 92
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