| Catalog No. | Size | Price (USD) |
| 10064-R027-50 | 50 µL | $177.58 |
| 10064-R027-100 | 100 µL | $340.51 |
General Information
| Product name | Recombinant Anti-BACE1 Antibody, Rabbit Monoclonal |
| Validated applications | FCM (FAQ Protocol), ICC/IF (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human BACE1 |
| Immunogen | Recombinant Human BACE1 protein (Catalog#10064-H08H) |
| Preparation | This antibody was obtained from a rabbit immunized with purified, recombinant Human BACE1 (rh BACE1; Catalog#10064-H08H; NP_036236.1; Met1-Thr457). |
| Source | Monoclonal Rabbit IgG Clone #027 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-ASP2 Antibody; Anti-BACE Antibody; Anti-HSPC104 Antibody; Anti-KIAA1149 Antibody
Image / Experimental Information
| Image 1 Description | Immunofluorescence staining of Human BACE1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human AK4 monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. |
| Image 1 Alt Text | Human BACE1 Immunofluorescence(IF) 3003 |
| Image 1 URL | Source image |
| Image 2 Description | Immunofluorescence staining of Human BACE1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human AK4 monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. |
| Image 2 Alt Text | Human BACE1 Flow Cytometry (FC) 5337 |
| Image 2 URL | Source image |
Background Information
| Full Name | beta-site APP-cleaving enzyme 1 |
| Description | Beta-site APP-cleaving enzyme 1 (BACE1) is an aspartic-acid protease important in the formation of myelin sheaths in peripheral nerve cells. In the brain, This protein is expressed highly in the substantia nigra, locus coruleus and medulla oblongata. Strong BACE1 expression has also been described in pancreatic tissue. BACE1 has a pivotal role in the pathogenesis of Alzheimer's disease. In Alzheimer's disease patients, BACE1 levels were elevated although mRNA levels were not changed. It has been found that BACE1 gene expression is controlled by a TATA-less promoter. The translational repression as a new mechanism controlling its expression. And the low concentrations of Ca(2+) (microM range) significantly increased the proteolytic activity of BACE1. Furthermore, BACE1 protein is ubiquitinated, and the degradation of BACE1 proteins and amyloid precursor protein processing are regulated by the ubiquitin-proteasome pathway. It has also been identified as the rate limiting enzyme for amyloid-beta-peptide (Abeta) production. |
| References |
|
title: Platinum-alginate-chitosan nanobioconjugate decorated carbon backbone layered biosensor for highly sensitive and selective detection of BACE-1.
authors: Sopan Nangare; Pravin Patil
journal: International Journal of Biological Macromolecules
date: 2023-10-01
pmid: 37558026
doi: 10.1016/j.ijbiomac.2023.126224
cited_by_count: 4
techniques: Recombinant
bioz_score: 91
title: Novel Multifunctional Ascorbic Triazole Derivatives for Amyloidogenic Pathway Inhibition, Anti-Inflammation, and Neuroprotection
authors: Jutamas Jiaranaikulwanitch; Hataichanok Pandith; ...; Opa Vajragupta
journal: Molecules
date: 2021-03-12
doi: 10.3390/molecules26061562
cited_by_count: 0
techniques: Activity Assay; Gene Expression; Liquid Chromatography with Mass Spectroscopy
bioz_score: 91
title: Lead discovery of a guanidinyl tryptophan derivative on amyloid cascade inhibition
authors: Piyapan Suwanttananuruk; Jutamas Jiaranaikulwanitch; ...; Opa Vajragupta
journal: Open Chemistry
date: 2020-06-09
doi: 10.1515/chem-2020-0067
cited_by_count: 3
techniques: ThT Assay
bioz_score: 91
