| Catalog No. | Size | Price (USD) |
| 101579-T32-50 | 50 µL | $147.96 |
| 101579-T32-100 | 100 µL | $251.64 |
| 101579-T32-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-BCL-xL/BCL2L1 Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human Mouse, Rat (Species predicted to react based on 100% sequence homology) |
| Immunogen | A synthetic peptide corresponding to the N-terminus of the Human BCL2L1 / Bcl-XL |
| Preparation | Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human BCL2L1 / Bcl-XL, and purified by antigen affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-Bcl-X Antibody; Anti-bcl-xL Antibody; Anti-BCL-XL/S Antibody; Anti-bcl-xS Antibody; Anti-BCL2L Antibody; Anti-BCLX Antibody; Anti-PPP1R52 Antibody
Image / Experimental Information
| Image 1 Description | Anti-BCL2L1 rabbit polyclonal antibody at 1:500 dilution Lane A: K562 Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: RAW264.7 Whole Cell Lysate Lane D: NIH-3T3 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:26 kDa Observed band size:32 kDa |
| Image 1 Alt Text | Human BCL2L1/Bcl-XL Western blot (WB) 6910 |
| Image 1 URL | Source image |
Background Information
| Full Name | BCL2-like 1 |
| Description | B-cell lymphoma-extra large (Bcl-xl) is a transmembrane molecule in the mitochondria. Bcl-xL (BCL2L1), belongs to the Bcl-2 family. Members of the bcl-2 family encode proteins that function either to promote or to inhibit apoptosis. Antiapoptotic members such as Bcl-2 and Bcl-xL prevent PCD in response to a wide variety of stimuli to take part in cancer survival. Conversely, proapoptotic proteins, exemplified by Bax and Bak, can accelerate death and in some instances are sufficient to cause apoptosis independent of additional signals. The crystal and solution structures of a Bcl-2 family member, Bcl-xL is like this: The structures consist of two central, primarily hydrophobic α-helices, which are surrounded by amphipathic helices. A 60-residue loop connecting helices αl and α2 was found to be flexible and non-essential for anti-apoptotic activity. Bcl-xL is characterized as an important factor in autophagy, inhibiting Beclin 1-mediated autophagy by binding to Beclin 1. In addition, Beclin 1, Bcl-2 and Bcl-xL can cooperate with Atg5 or Ca to regulate both autophagy and apoptosis. Bcl-xL is also implicated in anoxia induced cell death. The pathway is initiated by the loss of function of the prosurvival Bcl-2 family members Mcl-1 and Bcl-2 / Bcl-XL, resulting in Bax- or Bak-dependent release of cytochrome c and subsequent caspase-9-dependent cell death. Thus, Bcl-xL, the well-characterized apoptosis guards, appears to be important in cell death. |
| Related Pathways |
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| References |
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title: Growth of tyrosine kinase inhibitor-resistant Philadelphia-positive acute lymphoblastic leukemia: Role of bone marrow stromal cells
authors: Cheng Zhang; Xi Zhang; ...; Xing-Hua Chen
journal: Oncology Letters
date: 2017-04-01
pmid: 28454362
doi: 10.3892/ol.2017.5686
pmcid: 5403224
cited_by_count: 3
techniques: Blocking Assay; Bradford Assay; Cell Culture; Concentration Assay; Expressing; Incubation; SDS Page; Western Blot
bioz_score: 90
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