| Catalog No. | Size | Price (USD) |
| 10458-R515-50 | 50 µL | $177.58 |
| 10458-R515-100 | 100 µL | $340.51 |
General Information
| Product name | Recombinant Anti-Carbonic Anhydrase XIV/CA14 Antibody, Rabbit Monoclonal |
| Validated applications | ELISA (FAQ Protocol), IHC-P (FAQ Protocol), ICC/IF (FAQ Protocol) |
| Species reactivity | Reacts with: Human Not React with: Mouse, Rat |
| Specificity | Human Carbonic Anhydrase XIV/CA14 No cross-reactivity in ELISA with Human CARPX Human CA12 Mouse CA14 |
| Immunogen | Recombinant Human Carbonic Anhydrase XIV / CA14 Protein (Catalog#10458-H08H) |
| Preparation | This antibody was obtained from a rabbit immunized with purified, recombinant Human Carbonic Anhydrase XIV / CA14 (rh Carbonic Anhydrase XIV / CA14; Catalog#10458-H08H; NP_036245.1; Met 1-Met 290). |
| Source | Monoclonal Rabbit IgG Clone #515 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-CAXiV Antibody; Anti-UNQ690/PRO1335 Antibody
Image / Experimental Information
| Image 1 Description | Immunofluorescence staining of Human CA14 in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CA14 monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane. |
| Image 1 Alt Text | Human Carbonic Anhydrase XIV Immunofluorescence(IF) 2994 |
| Image 1 URL | Source image |
| Image 2 Description | Immunofluorescence staining of Human CA14 in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CA14 monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane. |
| Image 2 Alt Text | Human Carbonic Anhydrase XIV Immunohistochemistry(IHC) 4633 |
| Image 2 URL | Source image |
Background Information
| Full Name | carbonic anhydrase XIV |
| Description | The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. CAXIV is a member of CA family that showed an overall similarity of 29–46% to other active CA isozymes. The highest percentage similarity was with a transmembrane CA isoform, CAXII. The CAXIV was found high concentrations in human heart, brain, liver, and skeletal muscle but lower in the colon, small intestine, urinary bladder, and kidney. No CAXIV mRNA was seen in the salivary gland and pancreas. CAXIV is a likely candidate for the extracellular CA postulated to have an important role in modulating excitatory synaptic transmission in brain. |
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