Anti-CCDC134 Antibody, Rabbit Polyclonal sinobiological 13168-T52

$143.00
In stock
SKU
13168-T52
Catalog No.SizePrice (USD)
13168-T52-5050 µL$147.96
13168-T52-100100 µL$251.64
13168-T52-200200 µL$355.32

General Information

Product nameAnti-CCDC134 Antibody, Rabbit Polyclonal
Validated applicationsWB (FAQ Protocol), ELISA (FAQ Protocol), IP (FAQ Protocol)
Species reactivityReacts with: Human
SpecificityHuman CCDC134
ImmunogenRecombinant Human CCDC134 protein (Catalog#13168-H08H)
PreparationProduced in rabbits immunized with purified, recombinant Human CCDC134 (rh CCDC134; Catalog#13168-H08H; Q9H6E4; Met1-Leu229). CCDC134 specific IgG was purified by Human CCDC134 affinity chromatography.
SourcePolyclonal Rabbit IgG
PurificationProtein A & Antigen Affinity
Formulation0.2 μm filtered solution in PBS
ConjugateUnconjugated
FormLiquid
ShippingThis antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
StorageThis antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Synonyms: Anti-FLJ22349 Antibody; Anti-OI22 Antibody

Image / Experimental Information

Image 1 DescriptionCCDC134 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate Lane B:0.5 mg 293T Whole Cell Lysate 0.5 µL anti-CCDC134 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-CCDC134 rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 28 kDa Observed band size: 28 kDa
Image 1 Alt TextHuman CCDC134 Immunoprecipitation(IP) 6050
Image 1 URLSource image
Image 2 DescriptionCCDC134 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate Lane B:0.5 mg 293T Whole Cell Lysate 0.5 µL anti-CCDC134 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-CCDC134 rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 28 kDa Observed band size: 28 kDa
Image 2 Alt TextHuman CCDC134 Western blot (WB) 6679
Image 2 URLSource image

Background Information

Full Namecoiled-coil domain containing 134
DescriptionCoiled-coil domain containing 134 (CCDC134) is a 229 amino acids secretory protein. Coiled-coil domain is a motif in which alpha-helix are coiled together. It has been found in many types of proteins, including transcription factors, intermediate filaments and certain tRNA synthetases. Many proteins containing such motif CCDC134 are involved in important biological functions. CCDC134 is also considered as a novel human MAPK-regulating protein that can inhibit the MAPK pathway. This protein significantly inhibite Elk1 transcriptional activity. The coiled-coil domain is a ubiquitous protein motif that is often involved in oligomerization.
References
  1. Huang J, et al. (2007) CCDC134, a novel secretory protein, inhibits activation of ERK and JNK, but not p38 MAPK. Cellular and Molecular Life Sciences. 65(2): 338-49.
  2. Kim S, et al. (2011) Genome-wide association study of CSF biomarkers Abeta1-42, t-tau, and p-tau181p in the ADNI cohort. Neurology. 76(1): 69-79.

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