WB (FAQ Protocol), ELISA (FAQ Protocol), IP (FAQ Protocol)
Species reactivity
Reacts with: Human
Specificity
Human CCDC134
Immunogen
Recombinant Human CCDC134 protein (Catalog#13168-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human CCDC134 (rh CCDC134; Catalog#13168-H08H; Q9H6E4; Met1-Leu229). CCDC134 specific IgG was purified by Human CCDC134 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
CCDC134 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate Lane B:0.5 mg 293T Whole Cell Lysate 0.5 µL anti-CCDC134 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-CCDC134 rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 28 kDa Observed band size: 28 kDa
CCDC134 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate Lane B:0.5 mg 293T Whole Cell Lysate 0.5 µL anti-CCDC134 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-CCDC134 rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 28 kDa Observed band size: 28 kDa
Coiled-coil domain containing 134 (CCDC134) is a 229 amino acids secretory protein. Coiled-coil domain is a motif in which alpha-helix are coiled together. It has been found in many types of proteins, including transcription factors, intermediate filaments and certain tRNA synthetases. Many proteins containing such motif CCDC134 are involved in important biological functions. CCDC134 is also considered as a novel human MAPK-regulating protein that can inhibit the MAPK pathway. This protein significantly inhibite Elk1 transcriptional activity. The coiled-coil domain is a ubiquitous protein motif that is often involved in oligomerization.
References
Huang J, et al. (2007) CCDC134, a novel secretory protein, inhibits activation of ERK and JNK, but not p38 MAPK. Cellular and Molecular Life Sciences. 65(2): 338-49.
Kim S, et al. (2011) Genome-wide association study of CSF biomarkers Abeta1-42, t-tau, and p-tau181p in the ADNI cohort. Neurology. 76(1): 69-79.