| Catalog No. | Size | Price (USD) |
| 10115-MM05-1 | 1 mL | $1,377.43 |
| 10115-MM05-20 | 20 µL | $88.87 |
| 10115-MM05-50 | 50 µL | $147.96 |
| 10115-MM05-100 | 100 µL | $251.64 |
| 10115-MM05-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-CD146/MCAM Antibody, Mouse Monoclonal |
| Validated applications | ELISA (FAQ Protocol), ELISA(Cap) (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human CD146/MCAM No cross-reactivity in ELISA and WB with Human CD66a / CEACAM1 Human CD166 / alcama Human CD106 / VCAM1 Human CD226 / DNAM-1 Human BCAM / CD239 Human cell lysate (293 cell line). |
| Immunogen | Recombinant Human CD146 protein (Catalog#10115-H08H) |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD146 / MCAM extracellular domain (rh CD146; Catalog#10115-H08H; NP_006491.2; Met 1-Gly 559). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Source | Monoclonal Mouse IgG1 Clone #2E2F4D5 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-CD146 Antibody; Anti-HEMCAM Antibody; Anti-MelCAM Antibody; Anti-METCAM Antibody; Anti-MUC18 Antibody
Background Information
| Full Name | melanoma cell adhesion molecule |
| Description | The CD146 antigen, also known as melanoma cell adhesion molecule (MCAM) and MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region, and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. This protein is also expressed predominantly on metastatic lesions and advanced primary tumors, and thus has been suggested to play an important role in tumor progression and the development of metastasis in certain human carcinomas. |
| References |
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