| Catalog No. | Size | Price (USD) |
| 10115-R005-50 | 50 µL | $177.58 |
| 10115-R005-100 | 100 µL | $340.51 |
General Information
| Product name | Recombinant Anti-CD146/MCAM Antibody, Rabbit Monoclonal |
| Validated applications | WB (FAQ Protocol), IHC-P (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human CD146/MCAM No cross-reactivity in ELISA with Human CD66a / CEACAM1 Human CD166 / alcama Human CD106 / VCAM1 Human CD226 / DNAM-1 Human BCAM / CD239 |
| Immunogen | Recombinant Human CD146 protein (Catalog#10115-H08H) |
| Preparation | This antibody was obtained from a rabbit immunized with purified, recombinant Human CD146 / MCAM extracellular domain (rh CD146; Catalog#10115-H08H; NP_006491.2; Met 1-Gly 559) . |
| Source | Monoclonal Rabbit IgG Clone #005 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-CD146 Antibody; Anti-HEMCAM Antibody; Anti-MelCAM Antibody; Anti-METCAM Antibody; Anti-MUC18 Antibody
Image / Experimental Information
| Image 1 Description | Immunohistochemical staining of formalin fixed, paraffin-embedded human stomach showing membrane staining of endothelial cells (red arrow), smooth muscle cells (black arrow) (1:200). |
| Image 1 Alt Text | Human CD146/MCAM Immunohistochemistry(IHC) 2709 |
| Image 1 URL | Source image |
| Image 2 Description | Immunohistochemical staining of formalin fixed, paraffin-embedded human stomach showing membrane staining of endothelial cells (red arrow), smooth muscle cells (black arrow) (1:200). |
| Image 2 Alt Text | Human CD146/MCAM Immunohistochemistry(IHC) 2718 |
| Image 2 URL | Source image |
| Image 3 Description | Immunohistochemical staining of formalin fixed, paraffin-embedded human stomach showing membrane staining of endothelial cells (red arrow), smooth muscle cells (black arrow) (1:200). |
| Image 3 Alt Text | Human CD146/MCAM Immunohistochemistry(IHC) 2727 |
| Image 3 URL | Source image |
| Image 4 Description | Immunohistochemical staining of formalin fixed, paraffin-embedded human stomach showing membrane staining of endothelial cells (red arrow), smooth muscle cells (black arrow) (1:200). |
| Image 4 Alt Text | Human CD146/MCAM Western blot (WB) 6882 |
| Image 4 URL | Source image |
Background Information
| Full Name | melanoma cell adhesion molecule |
| Description | The CD146 antigen, also known as melanoma cell adhesion molecule (MCAM) and MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region, and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. This protein is also expressed predominantly on metastatic lesions and advanced primary tumors, and thus has been suggested to play an important role in tumor progression and the development of metastasis in certain human carcinomas. |
| References |
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