| Catalog No. | Size | Price (USD) |
| 10389-MM04-50 | 50 µL | $147.96 |
| 10389-MM04-100 | 100 µL | $251.64 |
| 10389-MM04-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-CD16a/FCGR3A/Fc gamma RIIIA Antibody, Mouse Monoclonal |
| Validated applications | IHC-P (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human CD16a/FCGR3A/Fc gamma RIIIA Has cross-reactivity in ELISA with Human CD16b No cross-reactivity in ELISA with Human CD32a Human CD32b Human CD64 |
| Immunogen | Recombinant Human CD16a / FCGR3A protein (Catalog#10389-H08H) |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD16a / FCGR3A (rh CD16a / FCGR3A; Catalog#10389-H08H; P08637-1; Met1-Gln208,176F). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Source | Monoclonal Mouse IgG1 Clone #04 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-CD16 Antibody; Anti-CD16-II Antibody; Anti-CD16A Antibody; Anti-FCG3 Antibody; Anti-FcgammaRIIIa Antibody; Anti-FCGR3 Antibody; Anti-FCGRIII Antibody; Anti-FcGRIIIA Antibody; Anti-FCR-10 Antibody; Anti-FCRIII Antibody; Anti-FCRIIIA Antibody; Anti-IGFR3 Antibody; Anti-IMD20 Antibody
Image / Experimental Information
| Image 1 Description | Immunochemical staining of human CD16a in human spleen with mouse monoclonal antibody (1:500, formalin-fixed paraffin embedded sections). |
| Image 1 Alt Text | Human CD16a/Fc gamma RIIIa Immunohistochemistry(IHC) 4554 |
| Image 1 URL | Source image |
| Image 2 Description | Immunochemical staining of human CD16a in human spleen with mouse monoclonal antibody (1:500, formalin-fixed paraffin embedded sections). |
| Image 2 Alt Text | Human CD16a/Fc gamma RIIIa Immunohistochemistry(IHC) 4555 |
| Image 2 URL | Source image |
| Image 3 Description | Immunochemical staining of human CD16a in human spleen with mouse monoclonal antibody (1:500, formalin-fixed paraffin embedded sections). |
| Image 3 Alt Text | Human CD16a/Fc gamma RIIIa Immunohistochemistry(IHC) 4556 |
| Image 3 URL | Source image |
Background Information
| Full Name | Fc fragment of IgG, low affinity IIIa, receptor (CD16a) |
| Description | The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells. CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation. |
| Research Areas |
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| References |
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title: An immune cell atlas reveals the dynamics of human macrophage specification during prenatal development.
authors: Zeshuai Wang; Zhisheng Wu; ...; Hanjie Li
journal: Cell
date: 2023-10-02
pmid: 37703875
doi: 10.1016/j.cell.2023.08.019
cited_by_count: 151
techniques: Immunohistochemistry; Polymer; Polymerase Chain Reaction; Real-time Polymerase Chain Reaction; Recombinant; Reverse Transcription; SYBR Green Assay
bioz_score: 91
title: Antitumor Efficacy of Anti-GD2 IgG1 Is Enhanced by Fc Glyco-Engineering
authors: Hong Xu; Hongfen Guo; ...; Nai-Kong V. Cheung
journal: Cancer Immunology Research
date: 2016-05-16
doi: 10.1158/2326-6066.cir-15-0221
cited_by_count: 17
techniques: Binding Assay; Flow Cytometry; In Vitro; Produced; Recombinant; SPR Assay
bioz_score: 91
