| Catalog No. | Size | Price (USD) |
| 11958-MM13-F-25 | 25 Tests | $103.68 |
| 11958-MM13-F-100 | 100 Tests | $325.84 |
General Information
| Product name | Anti-Siglec-2/CD22 Antibody (FITC), Mouse Monoclonal |
| Validated applications | FCM (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human Siglec-2/CD22 |
| Immunogen | Recombinant Human CD22 / Siglec-2 Protein (Catalog#11958-H08H) |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD22 / Siglec-2 (rh CD22 / Siglec-2; Catalog#11958-H08H; P20273-1; Met1-Arg687) and conjugated with FITC under optimum conditions, the unreacted FITC was removed. |
| Source | Monoclonal Mouse IgG1 Clone #13 |
| Purification | Protein A |
| Formulation | PBS solution containing 0.5% BSA and 0.09% sodium azide |
| Concentration | 5 μl/Test, 0.1 mg/ml |
| Conjugate | FITC |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal. |
Synonyms: Anti-SIGLEC-2 Antibody; Anti-SIGLEC2 Antibody
Image / Experimental Information
| Image 1 Description | Flow cytometric analysis of Human CD22 expression on human whole blood lymphocytes. Cells were stained with FITC-conjugated anti-Human CD22 and APC-conjugated anti-Human CD19. The dot plots were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. |
| Image 1 Alt Text | Human CD22/Siglec-2 Flow Cytometry (FC) 5285 |
| Image 1 URL | Source image |
Background Information
| Full Name | CD22 molecule |
| Description | CD22 is a member of the immunoglobulin superfamily, SIGLEC family of lectins. It is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. CD22 serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. In addition to its potential role as a mediator of intercellular interactions, signal transduction through CD22 can activate B cells and modulate antigen receptor signaling in vitro. The phenotype of CD22-deficient mice suggests that CD22 is primarily involved in the generation of mature B cells within the bone marrow, blood, and marginal zones of lymphoid tissues. CD22 recruits the tyrosine phosphatase Src homology 2 domain-containing phosphatase 1 (SHP-1) to immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and inhibits B-cell receptor (BCR)-induced Ca2+ signaling on normal B cells. CD22 interacts specifically with ligands carrying alpha2-6-linked sialic acids. As an inhibitory coreceptor of the B-cell receptor (BCR), CD22 plays a critical role in establishing signalling thresholds for B-cell activation. Like other coreceptors, the ability of CD22 to modulate B-cell signalling is critically dependent upon its proximity to the BCR, and this in turn is governed by the binding of its extracellular domain to alpha2,6-linked sialic acid ligands. However, genetic studies in mice reveal that some CD22 functions are regulated by ligand binding, whereas other functions are ligand-independent and may only require expression of an intact CD22 cytoplasmic domain at the B-cell surface. CD19 regulates CD22 phosphorylation by augmenting Lyn kinase activity, while CD22 inhibits CD19 phosphorylation via SHP-1. |
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