Recombinant Human CD300C protein (Catalog#11832-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD300C (rh CD300C; Catalog#11832-H08H; NP_006669.1; Met 1-Arg 183) and conjugated with APC under optimum conditions, the unreacted APC was removed.
Source
Monoclonal Mouse IgG1 Clone #03
Purification
Protein A
Formulation
PBS solution containing 0.5% BSA and 0.09% sodium azide
Concentration
5 μl/Test, 0.1 mg/ml
Conjugate
APC
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Flow cytometric analysis of anti-human CD300c on human whole blood monocytes. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable monocytes.
The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD300 is a glycoprotein family of cell surface molecules that regulate a diverse array of cellular processes via their triggering and inhibitory receptor functions. The CD300 family of myeloid immunoglobulin receptors includes activating(CD300b, CD300e) and inhibitory members(CD300a, CD300f), as well as CD300c and CD300d, whose function is uncertain.
Research Areas
ITIM/ITAM Immunoreceptors and Related Molecules
References
Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
Lankry D, et al. (2010) Expression and function of CD300 in NK cells.185(5): 2877-86.
Agueda MB, et al. (2010) CD300 heterocomplexes, a new and family-restricted mechanism for myeloid cell signaling regulation. Journal of biological chemistry. 285: 41781-94.
Clark GJ, et al. (2009) The CD300 molecules regulate monocyte and dendritic cell functions. 214 (9-10): 730-6.