| Catalog No. | Size | Price (USD) |
| 12211-MM02-50 | 50 µL | $147.96 |
| 12211-MM02-100 | 100 µL | $251.64 |
| 12211-MM02-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-CD44 Antibody, Mouse Monoclonal |
| Validated applications | FCM (FAQ Protocol), ICC/IF (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human CD44 |
| Immunogen | Recombinant Human CD44 protein (Catalog#12211-H08H) |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD44 (rh CD44; Catalog#12211-H08H; P16070-1; Met1-Pro220). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Source | Monoclonal Mouse IgG1 Clone #02 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-CD44R Antibody; Anti-CDW44 Antibody; Anti-CSPG8 Antibody; Anti-ECM-III Antibody; Anti-ECMR-III Antibody; Anti-H-CAM Antibody; Anti-HCELL Antibody; Anti-Hermes-1 Antibody; Anti-HUTCH-1 Antibody; Anti-HUTCH-I Antibody; Anti-IN Antibody; Anti-LHR Antibody; Anti-MC56 Antibody; Anti-MDU2 Antibody; Anti-MDU3 Antibody; Anti-MIC4 Antibody; Anti-PGP-1 Antibody; Anti-Pgp1 Antibody
Image / Experimental Information
| Image 1 Description | Immunofluorescence staining of Human CD44 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human CD44 monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane and cytoplasm. |
| Image 1 Alt Text | Human CD44 Immunofluorescence(IF) 2971 |
| Image 1 URL | Source image |
| Image 2 Description | Immunofluorescence staining of Human CD44 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human CD44 monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane and cytoplasm. |
| Image 2 Alt Text | Human CD44 Flow Cytometry (FC) 5256 |
| Image 2 URL | Source image |
Background Information
| Full Name | CD44 molecule (Indian blood group) |
| Description | CD44 is a type I transmembrane protein and a member of the cartilage link protein family. It is involved in cell-cell and cell-matrix interactions and signal transduction. Several CD44 ligands have been identified. The most extensively characterized ligand for CD44 is hyaluronan, a component of the extracellular matrix. CD44 protein is expressed on the majority of immune cells. The binding of CD44 to hyaluronan is induced on T lymphocytes after activation by antigen and on monocytes after stimulation by inflammatory agents. Under inflammatory conditions, CD44 on endothelial cells presents hyaluronan to CD44 on activated T lymphocytes and mediates a rolling interaction under flow conditions. Perturbations of the hyaluronan-CD44 interaction at the plasma membrane by various antagonists result in attenuation of receptor tyrosine kinase and transporter activities and inhibition of tumor progression in vivo. CD44 is known to interact with the ezrin family (ERM family) members and form a complex that plays diverse roles within both normal and abnormal cells, particularly cancer cells. CD44 and ezrin and their respective complex have properties suggesting that they may be important in the process of tumour-endothelium interactions, cell migrations, cell adhesion, tumour progression and metastasis. |
| References |
|
title: OTUD7B knockdown inhibits the proliferation and stemness of breast cancer cells by destabilizing FOXM1
authors: Hebing Wang; Sumei Han; ...; Dexiang Zhuo
journal: Oncology Letters
date: 2024-03-01
pmid: 38298430
pmcid: 10829069
cited_by_count: 2
techniques: Cell Counting; Expressing; Flow Cytometry; Fluorescence; Incubation; Negative Control; Over Expression; Software; Western Blot
bioz_score: 93
title: KK-LC-1 as a therapeutic target to eliminate ALDH + stem cells in triple negative breast cancer
authors: Jiawen Bu; Yixiao Zhang; ...; Caigang Liu
journal: Nature Communications
date: 2023-05-05
pmid: 37147285
doi: 10.1038/s41467-023-38097-1
pmcid: 10163259
cited_by_count: 25
techniques: Activity Assay; FACS; Flow Cytometry; Fluorescence; Isolation; Multiple Displacement Amplification; Software; Staining
bioz_score: 93
title: Design of Cefotaxime Sodium-Loaded Polydopamine Coatings with Controlled Surface Roughness for Titanium Implants.
authors: Fei He; Jing Li; ...; Ping Zhou
journal: ACS Biomaterials Science Engineering
date: 2022-11-15
pmid: 36191062
doi: 10.1021/acsbiomaterials.2c00702
cited_by_count: 5
techniques: Control; Expressing; Flow Cytometry; Incubation; Saline; Software
bioz_score: 90
title: Establishment and Characterization of a Highly Metastatic Ovarian Cancer Cell Line
authors: Jiang Ruibin; Cheng Guoping; ...; Gu Linhui
journal: BioMed Research International
date: 2018-06-25
pmid: 30046596
doi: 10.1155/2018/3972534
pmcid: 6036838
cited_by_count: 7
techniques: Flow Cytometry
bioz_score: 90
