| Catalog No. | Size | Price (USD) |
| 10333-MM02-A-25 | 25 Tests | $133.30 |
| 10333-MM02-A-100 | 100 Tests | $414.71 |
General Information
| Product name | Anti-ICAM-3/CD50 Antibody (APC), Mouse Monoclonal |
| Validated applications | FCM (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human ICAM-3/CD50 |
| Immunogen | Recombinant Human ICAM3 / CD50 protein (Catalog#10333-H08H) |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD50 / ICAM-3 (rh CD50 / ICAM-3; Catalog#10333-H08H; CAA49369.1; Met 1-His 485) and conjugated with APC under optimum conditions, the unreacted APC was removed. |
| Source | Monoclonal Mouse IgG2a Clone #02 |
| Purification | Protein A |
| Formulation | PBS solution containing 0.5% BSA and 0.09% sodium azide |
| Concentration | 5 μl/Test, 0.1 mg/ml |
| Conjugate | APC |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal. |
Synonyms: Anti-CD50 Antibody; Anti-CDW50 Antibody; Anti-ICAM-R Antibody
Image / Experimental Information
| Image 1 Description | Profile of peripheral blood lymphocytes analyzed by flow cytometry. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphocytes. |
| Image 1 Alt Text | Human ICAM-3 / CD50 Flow Cytometry (FC) 2537 |
| Image 1 URL | Source image |
Background Information
| Full Name | intercellular adhesion molecule 3 |
| Description | The protein ICAM-3, also known as CD50, is a member of the intercellular adhesion molecule (ICAM) family consisting of three members. It is a DC-SIGN ligand that is constitutively expressed on resting leukocytes and is thus an important molecule for the first immune response. ICAM-3 comprises five immunoglobulin-like domains and binds LFA-1 through its two N-terminal domains. It functions not only as an adhesion molecule but also as a potent signaling molecule. ICAM-3 binds to LFA-1 on antigen-presenting cells (APC) stabilizing the T cell-APC interaction, facilitating signaling through the CD3/TCR complex. However, recent evidence using cultured and transformed T cells suggests ICAM-3 may also function in signaling. It has been reported that the CD50 molecule can play a role in developing functionally mature T lymphocytes and its expression increases during the maturation process of T lymphocytes. Also, the interactions of ICAM-3 and LFA-1 facilitate HIV-1- induced virological synapse formation between T cells. ICAM-3 is associated with an increase in cellular radio-resistance and cancer cell proliferation. It could be considered as a candidate for anti-cancer drug development and as a cancer diagnostic marker. |
| References |
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