| Catalog No. | Size | Price (USD) |
| 10333-RP02-50 | 50 µL | $147.96 |
| 10333-RP02-100 | 100 µL | $251.64 |
| 10333-RP02-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-ICAM-3/CD50 Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), ELISA (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human ICAM-3/CD50 |
| Immunogen | Recombinant Human ICAM3 / CD50 protein (Catalog#10333-H08H) |
| Preparation | Produced in rabbits immunized with purified, recombinant Human ICAM3 / CD50 (rh ICAM3 / CD50; Catalog#10333-H08H; CAA49369.1; Met 1-His 485). ICAM3 / CD50 specific IgG was purified by Human ICAM3 / CD50 affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-CD50 Antibody; Anti-CDW50 Antibody; Anti-ICAM-R Antibody
Image / Experimental Information
| Image 1 Description | Anti-ICAM3 rabbit polyclonal antibody at 1:500 dilution Lane A: HL-60 Membrane Lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:60 kDa Observed band size:150 kDa |
| Image 1 Alt Text | Human ICAM-3 / CD50 Western blot (WB) 13187 |
| Image 1 URL | Source image |
| Image 2 Description | Anti-ICAM3 rabbit polyclonal antibody at 1:500 dilution Lane A: HL-60 Membrane Lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:60 kDa Observed band size:150 kDa |
| Image 2 Alt Text | Human CD50 Immunoprecipitation(IP) 30542 |
| Image 2 URL | Source image |
Background Information
| Full Name | intercellular adhesion molecule 3 |
| Description | The protein ICAM-3, also known as CD50, is a member of the intercellular adhesion molecule (ICAM) family consisting of three members. It is a DC-SIGN ligand that is constitutively expressed on resting leukocytes and is thus an important molecule for the first immune response. ICAM-3 comprises five immunoglobulin-like domains and binds LFA-1 through its two N-terminal domains. It functions not only as an adhesion molecule but also as a potent signaling molecule. ICAM-3 binds to LFA-1 on antigen-presenting cells (APC) stabilizing the T cell-APC interaction, facilitating signaling through the CD3/TCR complex. However, recent evidence using cultured and transformed T cells suggests ICAM-3 may also function in signaling. It has been reported that the CD50 molecule can play a role in developing functionally mature T lymphocytes and its expression increases during the maturation process of T lymphocytes. Also, the interactions of ICAM-3 and LFA-1 facilitate HIV-1- induced virological synapse formation between T cells. ICAM-3 is associated with an increase in cellular radio-resistance and cancer cell proliferation. It could be considered as a candidate for anti-cancer drug development and as a cancer diagnostic marker. |
| References |
|
-HB04NO0907-WB.png){kind=link}
