| Catalog No. | Size | Price (USD) |
| 12409-MM05-C-25 | 25 Tests | $133.30 |
| 12409-MM05-C-100 | 100 Tests | $325.84 |
General Information
| Product name | Anti-CD58/LFA-3 Antibody (PerCP), Mouse Monoclonal |
| Validated applications | FCM (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human CD58/LFA-3 |
| Immunogen | Recombinant Human LFA-3 / CD58 Protein (Catalog#12409-H08H) |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human LFA-3 / CD58 (rh LFA-3 / CD58; Catalog#12409-H08H; AAH05930.1; Met1-Arg215) and conjugated with PerCP under optimum conditions, the unreacted PerCP was removed. |
| Source | Monoclonal Mouse IgG1 Clone #05 |
| Purification | Protein A |
| Formulation | PBS solution containing 0.5% BSA and 0.09% sodium azide |
| Concentration | 10 μl/Test, 0.1 mg/ml |
| Conjugate | PerCP |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal. |
Synonyms: Anti-ag3 Antibody; Anti-LFA-3 Antibody; Anti-LFA3 Antibody
Image / Experimental Information
| Image 1 Description | Flow cytometric analysis of Human CD58 expression on human whole blood lymphocytes. Cells were stained with PerCP-conjugated anti-Human CD58. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. |
| Image 1 Alt Text | Human LFA-3 / CD58 Flow Cytometry (FC) 15900 |
| Image 1 URL | Source image |
Background Information
| Full Name | CD58 molecule |
| Description | CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a 65–70 kDa glycoprotein belonging to the CD2 family of the immunoglobulin superfamily. It serves as the sole ligand for CD2, a receptor primarily expressed on T cells, NK cells, and dendritic cells. CD58 exists in two membrane-anchored forms—transmembrane and GPI-linked—and is widely distributed across both hematopoietic and non-hematopoietic tissues, including leukocytes, erythrocytes, endothelial cells, epithelial cells, and fibroblasts. The CD2–CD58 interaction plays a central role in the immunological synapse, mediating T cell adhesion, co-stimulatory activation, cytokine production, and cytotoxic activity. A soluble form of CD58 (sCD58) has also been identified, which acts as an immunosuppressive factor by disrupting CD2–CD58 binding. |
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