| Catalog No. | Size | Price (USD) |
| 12409-R085-50 | 50 µL | $177.58 |
| 12409-R085-100 | 100 µL | $340.51 |
General Information
| Product name | Recombinant Anti-CD58/LFA-3 Antibody, Rabbit Monoclonal |
| Validated applications | WB (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human CD58/LFA-3 |
| Immunogen | Recombinant Human LFA-3 / CD58 Protein (Catalog#12409-H08H) |
| Preparation | This antibody was obtained from a rabbit immunized with purified, recombinant Human LFA-3 / CD58 (rh LFA-3 / CD58; Catalog#12409-H08H; AAH05930.1; Met1-Arg215). |
| Source | Monoclonal Rabbit IgG Clone #085 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-ag3 Antibody; Anti-LFA-3 Antibody; Anti-LFA3 Antibody
Image / Experimental Information
| Image 1 Description | Anti-CD58 rabbit monoclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: K562 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:61 kDa (We are unsure as to the identity of these extra bands.) |
| Image 1 Alt Text | Human LFA-3 / CD58 Western blot (WB) 16056 |
| Image 1 URL | Source image |
| Image 2 Description | Anti-CD58 rabbit monoclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: K562 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:61 kDa (We are unsure as to the identity of these extra bands.) |
| Image 2 Alt Text | Human LFA-3 / CD58 Immunoprecipitation(IP) 16058 |
| Image 2 URL | Source image |
Background Information
| Full Name | CD58 molecule |
| Description | CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a 65–70 kDa glycoprotein belonging to the CD2 family of the immunoglobulin superfamily. It serves as the sole ligand for CD2, a receptor primarily expressed on T cells, NK cells, and dendritic cells. CD58 exists in two membrane-anchored forms—transmembrane and GPI-linked—and is widely distributed across both hematopoietic and non-hematopoietic tissues, including leukocytes, erythrocytes, endothelial cells, epithelial cells, and fibroblasts. The CD2–CD58 interaction plays a central role in the immunological synapse, mediating T cell adhesion, co-stimulatory activation, cytokine production, and cytotoxic activity. A soluble form of CD58 (sCD58) has also been identified, which acts as an immunosuppressive factor by disrupting CD2–CD58 binding. |
| Tags |
|
| References |
|
