| Catalog No. | Size | Price (USD) |
| 11280-RP02-50 | 50 µL | $147.96 |
| 11280-RP02-100 | 100 µL | $251.64 |
| 11280-RP02-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-CD97 Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), ELISA (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human CD97 |
| Immunogen | Recombinant Human CD97 protein (Catalog#11280-H08H) |
| Preparation | Produced in rabbits immunized with purified, recombinant Human CD97 (rh CD97; Catalog#11280-H08H; NP_001775.2; Met 1-Gln 398). CD97 specific IgG was purified by human CD97 affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-CD97 Antibody; Anti-TM7LN1 Antibody
Image / Experimental Information
| Image 1 Description | CD97 was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg SW480 Whole Cell Lysate 0.5 µL anti-CD97 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-CD97 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 92 kDa Observed band size: 92 kDa |
| Image 1 Alt Text | Human CD97 Immunoprecipitation(IP) 8077 |
| Image 1 URL | Source image |
| Image 2 Description | CD97 was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg SW480 Whole Cell Lysate 0.5 µL anti-CD97 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-CD97 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 92 kDa Observed band size: 92 kDa |
| Image 2 Alt Text | Human CD97 Western blot (WB) 13383 |
| Image 2 URL | Source image |
Background Information
| Full Name | adhesion G protein-coupled receptor E5 |
| Description | The cluster of differentiation (CD) system is commonly used as cell markers in immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 32 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. The CD97 is a receptor predominantly expressed in leukocytes and belongs to a new group of seven-span transmembrane molecules, which is also designed EGF-TM7 family. The family members are characterized by an extended extracellular region with several N-terminal epidermal growth factor-like domains two of which contain a calcium-binding site. Mature CD 97 has two noncovalently associated subunits and is composed of a large extracellular protein (CD97 alpha) and a seven-membrane spanning protein (CD97 beta). CD97 is considered as a defining feature of G protein-coupled receptors. The effects that lymphocytes and erythrocytes adhere to CD97-transfected COS cells suggest that CD97 has the ability to bind cellular ligands. CD97 alpha has three alternatively spliced isoforms that are related to the calcium-binding EGF-like repeats in the microfibril protein fibrillin. Leukocytes strongly positive for CD97 are concentrated at sites of inflammation relative to CD97 expression in normal lymphoid tissues. |
| References |
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