| Catalog No. | Size | Price (USD) |
| 105166-T32-50 | 50 µL | $147.96 |
| 105166-T32-100 | 100 µL | $251.64 |
| 105166-T32-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-Cdc25B Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human Cdc25B |
| Immunogen | A synthetic peptide corresponding to the center region of the Human Cdc25B |
| Preparation | Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Cdc25B, and purified by antigen affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-CDC25HU2 Antibody
Image / Experimental Information
| Image 1 Description | Anti-CDC25B rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:64 kDa |
| Image 1 Alt Text | Human Cdc25B Western blot (WB) 19752 |
| Image 1 URL | Source image |
Background Information
| Description | CDC25 phosphatases are key cell cycle regulators and represent very attractive but challenging targets for anticancer drug discovery. CDC25B may be used as a potential prognostic marker in the pathogenesis of retinoblastoma. In mitosis, Kiz is a new substrate of CDC25B whose dephosphorylation following CDC25B stabilization leads to the formation of multipolar spindles. Furthermore, endogenous Kiz and CDC25B interact only in mitosis, suggesting that Kiz phosphorylation depends on a balance between CDC25B and Plk1 activities. The abnormal expression of CDC25B phosphatases detected in a number of tumors implies that their dysregulation is involved in malignant transformation. Both CDC25B and PED/PEA-15 play a certain role in the carcinogenesis of esophageal cancer, they will be the new diagnostic/therapeutic targets in esophageal carcinoma. |
| Research Areas |
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