| Catalog No. | Size | Price (USD) |
| 100256-MM02-50 | 50 µL | $147.96 |
| 100256-MM02-100 | 100 µL | $251.64 |
| 100256-MM02-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-Chromogranin A/CHGA Antibody, Mouse Monoclonal |
| Validated applications | IHC-P (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human Chromogranin A/CHGA |
| Immunogen | A synthetic peptide corresponding to the N-terminus of the Human Chromogranin A |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the N-terminus of the Human Chromogranin A. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Source | Monoclonal Mouse IgG1 Clone #02 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-CGA Antibody; Anti-PHE5 Antibody; Anti-PHES Antibody
Image / Experimental Information
| Image 1 Description | Immunochemical staining of human ChromograninA in human pancreas with mouse monoclonal antibody (1:100, formalin-fixed paraffin embedded sections). |
| Image 1 Alt Text | Human Chromogranin A Immunohistochemistry(IHC) 4418 |
| Image 1 URL | Source image |
| Image 2 Description | Immunochemical staining of human ChromograninA in human pancreas with mouse monoclonal antibody (1:100, formalin-fixed paraffin embedded sections). |
| Image 2 Alt Text | Human Chromogranin A Immunohistochemistry(IHC) 4420 |
| Image 2 URL | Source image |
Background Information
| Full Name | chromogranin A |
| Description | The acidic glycoprotein chromogranin A (CHGA) is co-stored/co-secreted with catecholamines and crucial for secretory vesicle biogenesis in neuronal/neuroendocrine cells. CHGA is dysregulated in several cardiovascular diseases, but the underlying mechanisms are not well established. The CHGA promoter haplotype 2 (occurring in a large proportion of the world population) in enhancing CHGA expression in haplotype 2 carriers who may be at higher risk for cardiovascular/metabolic disorders. Chromogranin A (CHGA) plays a fundamental role in the biogenesis of catecholamine secretory granules. Changes in storage and release of CHGA in clinical and experimental hypertension prompted us to study whether genetic variation at the CHGA locus might contribute to alterations in autonomic function, and hence hypertension and its target organ consequences such as hypertensive renal disease (nephrosclerosis). Chromogranin A (CHGA) plays a catalytic role in formation of catecholamine storage vesicles and also serves as precursor to the peptide fragment catestatin, a catecholamine secretory inhibitor whose expression is diminished in the hypertensive individuals. The chromogranin/secretogranin proteins are costored and coreleased with catecholamines from secretory vesicles in chromaffin cells and noradrenergic neurons. Chromogranin A (CHGA) regulates catecholamine storage and release through intracellular (vesiculogenic) and extracellular (catecholamine release-inhibitory) mechanisms. CHGA is a candidate gene for autonomic dysfunction syndromes, including intermediate phenotypes that contribute to human hypertension. |
