| Catalog No. | Size | Price (USD) |
| 100312-MM03-50 | 50 µL | $147.96 |
| 100312-MM03-100 | 100 µL | $251.64 |
| 100312-MM03-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-Cytokeratin 5/KRT5 Antibody, Mouse Monoclonal |
| Validated applications | WB (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human Cytokeratin 5/KRT5 |
| Immunogen | A synthetic peptide corresponding to the center region of the human KRT5 |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the center region of the human KRT5. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Source | Monoclonal Mouse IgG1 Clone #03 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-CK-5 Antibody; Anti-CK5 Antibody; Anti-DDD Antibody; Anti-DDD1 Antibody; Anti-EBS1 Antibody; Anti-EBS2 Antibody; Anti-EBS2A Antibody; Anti-EBS2B Antibody; Anti-EBS2C Antibody; Anti-EBS2D Antibody; Anti-EBS2E Antibody; Anti-EBS2F Antibody; Anti-K5 Antibody; Anti-KRT5A Antibody
Image / Experimental Information
| Image 1 Description | Anti-KRT5 mouse monoclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:62 kDa Observed band size:62 kDa |
| Image 1 Alt Text | Human KRT5/Cytokeratin5 Western blot (WB) 6456 |
| Image 1 URL | Source image |
Background Information
| Full Name | keratin 5, type II |
| Description | Keratin 5 is the major type II keratin of the basal cells of the epidermis and other stratified epithelia. With its type I partner, keratin 14, it constitutes a major fraction of the cytoskeleton of the basal cells. Epidermolysis bullosa simplex with mottled pigmentation (EBS-MP) is a rare dermatologic disorder of autosomal dominant inheritance with intraepidermal blistering after minor trauma, reticular hyperpigmentation unrelated to the blistering, nail dystrophy, and mild palmoplantar keratosis. Keratin 5 and keratin 14 are known to be essential for the basal keratinocyte cytoskeleton and are defective in several forms of epidermolysis bullosa simplex. Recently, a 71C-->T transition in the keratin 5 gene (KRT5) causing a P24L substitution was identified in some patients with EBS-MP. |
title: The development and characterization of in vivo-like three-dimensional models of bronchial epithelial cell lines.
authors: Sara Van den Bossche; Lisa Ostyn; ...; Aurélie Crabbé
journal: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences
date: 2023-11-01
pmid: 37633341
doi: 10.1016/j.ejps.2023.106567
cited_by_count: 8
techniques: Binding Assay; Blocking Assay; Control; Immunostaining; Incubation; Marker; Saline; Staining
bioz_score: 91
