| Catalog No. | Size | Price (USD) |
| 10730-RP02-50 | 50 µL | $147.96 |
| 10730-RP02-100 | 100 µL | $251.64 |
| 10730-RP02-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-DDR1 Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), ELISA (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human DDR1 |
| Immunogen | Recombinant Human DDR1 protein (Catalog#10730-H08H) |
| Preparation | Produced in rabbits immunized with purified, recombinant Human DDR1 / CD167a (rh DDR1; Catalog#10730-H08H; NP_001945.3; Met 1-Thr 416). DDR1 specific IgG was purified by human DDR1 affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-CAK Antibody; Anti-CD167 Antibody; Anti-DDR Antibody; Anti-EDDR1 Antibody; Anti-HGK2 Antibody; Anti-MCK10 Antibody; Anti-NEP Antibody; Anti-NTRK4 Antibody; Anti-PTK3 Antibody; Anti-PTK3A Antibody; Anti-RTK6 Antibody; Anti-TRKE Antibody
Image / Experimental Information
| Image 1 Description | DDR1 was immunoprecipitated using: Lane A:0.5 mg SW480 Whole Cell Lysate Lane B:0.5 mg MCF-7 Whole Cell Lysate 0.5 µL anti-DDR1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-DDR1 rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 101 kDa Observed band size: 130 kDa |
| Image 1 Alt Text | Human DDR1/MCK10/CD167 Immunoprecipitation(IP) 8013 |
| Image 1 URL | Source image |
| Image 2 Description | DDR1 was immunoprecipitated using: Lane A:0.5 mg SW480 Whole Cell Lysate Lane B:0.5 mg MCF-7 Whole Cell Lysate 0.5 µL anti-DDR1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-DDR1 rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 101 kDa Observed band size: 130 kDa |
| Image 2 Alt Text | Human DDR1/MCK10/CD167 Western blot (WB) 13268 |
| Image 2 URL | Source image |
Background Information
| Full Name | discoidin domain receptor tyrosine kinase 1 |
| Description | Discoidin domain receptor family, member 1 (DDR1), also known as or CD167a (cluster of differentiation 167a), and Mammary carcinoma kinase 10 (MCK10), belongs to a subfamily of tyrosine kinase receptors with an extracellular domain homologous to Dictyostellium discoideum protein discoidin 1. Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. Expression of DDR1/MCK10/CD167 is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. DDR1/MCK10/CD167 plays an important role in regulating attachment to collagen, chemotaxis, proliferation, and MMP production in smooth muscle cells. DDR1 functions in a feedforward loop to increase p53 levels and at least some of its effectors. Inhibition of DDR1 function resulted in strikingly increased apoptosis of wild-type p53-containing cells in response to genotoxic stress through a caspase-dependent pathway. |
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