| Catalog No. | Size | Price (USD) |
| 10605-R001-200 | 200 µg | $296.22 |
| 10605-R001-500 | 500 µg | $444.33 |
General Information
| Product name | EGF / Epidermal Growth Factor Neutralizing Antibody |
| Validated applications | Neutralization |
| Species reactivity | Reacts with: Human |
| Specificity | Human EGF Has cross-reactivity in ELISA with Canine EGF (Catalog # 170013-D07E) Mouse EGF (Catalog # 50482-MNCH) |
| Immunogen | Recombinant Human EGF / Epidermal Growth Factor Protein (Catalog#10605-HNAE) |
| Preparation | This antibody was obtained from a rabbit immunized with purified, recombinant Human EGF / Epidermal Growth Factor (rh EGF / Epidermal Growth Factor; Catalog#10605-HNAE; NP_001954.2; Asn971-Arg1023) and was produced using recombinant antibody technology. |
| Source | Monoclonal Rabbit IgG Clone #R001 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in Histidine and Arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH6.0 |
| Conjugate | Unconjugated |
| Endotoxin | < 3 EU/mg |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-HOMG4 Antibody; Anti-URG Antibody
Image / Experimental Information
| Image 1 Description | Cell Proliferation Induced by EGF was Neutralized by Human EGF Antibody. Recombinant Human EGF stimulates proliferation in the Balb/c 3T3 mouse embryonic fibroblast cell line. Proliferation elicited by Recombinant Human EGF (2 ng/mL) is neutralized by increasing concentrations of Human EGF Monoclonal Antibody (Catalog # 10605-R001). The IC50 is typically 2.5-7.5 μg/mL. |
| Image 1 Alt Text | Human Epidermal Growth Factor/EGF Blocking/Neutralization (B/N) 5601 |
| Image 1 URL | Source image |
Background Information
| Full Name | epidermal growth factor |
| Description | EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. EGF contains 9 EGF-like domains and 9 LDL-receptor class B repeats. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. As a low-molecular-weight polypeptide, EGF was first purified from the mouse submandibular gland, but since then it was found in many human tissues including submandibular gland, parotid gland. It can also be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is a growth factor that stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. It results in cellular proliferation, differentiation, and survival. Salivary EGF, which seems also regulated by dietary inorganic iodine, also plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity. EGF acts by binding with high affinity to epidermal growth factor receptor on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. The tyrosine kinase activity, in turn, initiates a signal transduction cascade that results in a variety of biochemical changes within the cell - a rise in intracellular calcium levels, increased glycolysis and protein synthesis, and increases in the expression of certain genes including the gene for EGFR - that ultimately lead to DNA synthesis and cell proliferation. |
| Related Pathways |
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| References |
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title: RIZ2 at the crossroad of the EGF/EGFR signaling in colorectal cancer
authors: Marzia Di Donato; Erika Di Zazzo; ...; Ciro Abbondanza
journal: Journal of Translational Medicine
date: 2023-10-18
pmid: 37853459
pmcid: 10585774
cited_by_count: 6
techniques: Cell Culture; Concentration Assay; Enzyme-linked Immunosorbent Assay; Immunofluorescence; Microscopy; Migration; Software; Staining; Transfection; Western Blot
bioz_score: 91
title: Cooperative regulation of adherens junction expansion through epidermal growth factor receptor activation.
authors: Chaoyu Fu; Aditya Arora; ...; Virgile Viasnoff
journal: Journal of cell science
date: 2022-03-08
pmid: 35099018
doi: 10.1242/jcs.258929
cited_by_count: 5
techniques: Control; Recombinant; Western Blot
bioz_score: 91
title: Analysis of Molecular Cargos in Single Extracellular Vesicle for Identification of Disease-Relevant Vesicles as Liquid Biopsy Markers
authors: Guo, Kaizhu
date: 2021-01-01
cited_by_count: 0
bioz_score: 91
title: Modulation of hepatitis B virus infection by epidermal growth factor secreted from liver sinusoidal endothelial cells
authors: Shin-Wei Chen; Misao Himeno; ...; Taketomo Kido
journal: Scientific Reports
date: 2020-09-01
pmid: 32873852
doi: 10.1038/s41598-020-71453-5
pmcid: 7462976
cited_by_count: 17
techniques: Blocking Assay; Immunofluorescence; Incubation; Inhibition; Negative Control; Staining; Western Blot
bioz_score: 91
