| Catalog No. | Size | Price (USD) |
| 14553-MM01-50 | 50 µL | $147.96 |
| 14553-MM01-100 | 100 µL | $251.64 |
| 14553-MM01-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-eIF3K Antibody, Mouse Monoclonal |
| Validated applications | ICC/IF (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human eIF3K |
| Immunogen | Recombinant Human EIF3K Protein (Catalog#14553-H20B) |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EIF3K (rh EIF3K; Catalog#14553-H20B; Q9UBQ5; Ala2-Gln218). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Source | Monoclonal Mouse IgG1 Clone #01 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-ARG134 Antibody; Anti-EIF3-p28 Antibody; Anti-EIF3S12 Antibody; Anti-HSPC029 Antibody; Anti-M9 Antibody; Anti-MSTP001 Antibody; Anti-PLAC-24 Antibody; Anti-PLAC24 Antibody; Anti-PRO1474 Antibody; Anti-PTD001 Antibody
Image / Experimental Information
| Image 1 Description | Immunofluorescence staining of Human EIF3K in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human EIF3K monoclonal antibody (1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green). Positive staining was localized to cytoplasm and nucleus. |
| Image 1 Alt Text | Human EIF3K Immunofluorescence(IF) 2937 |
| Image 1 URL | Source image |
Background Information
| Full Name | eukaryotic translation initiation factor 3, subunit K |
| Description | EIF3K is a member of the eIF3 subunit K family. It is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 4S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). It stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. EIF3K is universally expressed in human tissues. It is distributed both in nucleus and cytoplasm. EIF3K is the smallest subunit of eIF3 and it interacts with a number of other subunits of eIF3 and the 4S ribosomal subunit. |
| References |
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